Detailed information of TA system TA144857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 555619..556147
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: GPW86_RS02930
• Protein ID: WP_000221352.1
• Coordinates: 555619..555909 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0Q0JPP0
• Locus tag: GPW86_RS02935
• Protein ID: WP_019829909.1
• Coordinates: 555899..556147 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS02885	551041..551146	+	106	Protein_547	DUF645 family protein	-
GPW86_RS02890	551325..551840	+	516	WP_000090011.1	hypothetical protein	-
GPW86_RS02895	551960..552475	+	516	WP_000090011.1	hypothetical protein	-
GPW86_RS02905	552691..553608	+	918	WP_076025842.1	alpha/beta hydrolase	-
GPW86_RS19370	553964..554060	+	97	Protein_551	DUF645 family protein	-
GPW86_RS02920	554376..554801	+	426	WP_114721771.1	hypothetical protein	-
GPW86_RS02925	555037..555360	+	324	WP_080479241.1	DUF3709 domain-containing protein	-
GPW86_RS02930	555619..555909	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW86_RS02935	555899..556147	-	249	WP_019829909.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW86_RS02940	556663..556825	+	163	Protein_556	DUF645 family protein	-
GPW86_RS02945	557023..557415	+	393	WP_123011652.1	nuclear transport factor 2 family protein	-
GPW86_RS02960	558045..558512	+	468	WP_043992912.1	GNAT family N-acetyltransferase	-
GPW86_RS02965	558681..559184	+	504	WP_095467781.1	DinB family protein	-
GPW86_RS02970	559366..559596	-	231	WP_000838533.1	PAS factor family protein	-
GPW86_RS02975	559830..560507	+	678	WP_123011653.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	444653..571022	126369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T144857 WP_000221352.1 NZ_CP046741:c555909-555619 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T144857 NZ_CP062243:c4525077-4524820 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8967.31 Da
• Isoelectric Point: 4.1079

>AT144857 WP_019829909.1 NZ_CP046741:c556147-555899 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAITKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT144857 NZ_CP062243:c4524808-4524263 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A0Q0JPP0