Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 539565..540105 Replicon chromosome
Accession NZ_CP046741
Organism Vibrio cholerae strain 3523-03

Toxin (Protein)


Gene name relE Uniprot ID A0A151JGK8
Locus tag GPW86_RS02765 Protein ID WP_000277238.1
Coordinates 539565..539834 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KML3
Locus tag GPW86_RS02770 Protein ID WP_001258569.1
Coordinates 539827..540105 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW86_RS02715 534680..535075 + 396 WP_000870864.1 DUF3465 domain-containing protein -
GPW86_RS02720 535222..535671 + 450 WP_057556343.1 GyrI-like domain-containing protein -
GPW86_RS02725 535658..535786 + 129 WP_080390558.1 DUF3265 domain-containing protein -
GPW86_RS02730 535864..536379 + 516 WP_000284464.1 hypothetical protein -
GPW86_RS02735 537022..537273 + 252 WP_123011643.1 hypothetical protein -
GPW86_RS02740 537270..537485 + 216 WP_123011644.1 hypothetical protein -
GPW86_RS02745 537634..538341 + 708 WP_001893912.1 HAD family hydrolase -
GPW86_RS02750 538467..538733 + 267 WP_000589156.1 BrnT family toxin -
GPW86_RS02755 538720..538965 + 246 WP_000643600.1 hypothetical protein -
GPW86_RS02760 539143..539427 + 285 WP_000048765.1 antibiotic biosynthesis monooxygenase -
GPW86_RS02765 539565..539834 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin Toxin
GPW86_RS02770 539827..540105 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GPW86_RS02775 540588..540638 + 51 Protein_531 DUF645 family protein -
GPW86_RS02785 540921..541208 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin -
GPW86_RS02790 541219..541536 + 318 WP_001232701.1 HigA family addiction module antidote protein -
GPW86_RS19365 541837..542035 + 199 Protein_534 DUF645 family protein -
GPW86_RS02805 542377..542556 + 180 WP_032470611.1 DUF645 family protein -
GPW86_RS02810 542798..543121 + 324 WP_080476600.1 DUF3709 domain-containing protein -
GPW86_RS02815 543440..543955 + 516 WP_000284464.1 hypothetical protein -
GPW86_RS02820 544012..544206 + 195 WP_123011647.1 DUF3709 domain-containing protein -
GPW86_RS02825 544242..544460 + 219 WP_184476335.1 DUF3709 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 443489..637173 193684
- inside Integron - - 444653..571022 126369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10406.11 Da        Isoelectric Point: 9.5084

>T144855 WP_000277238.1 NZ_CP046741:c539834-539565 [Vibrio cholerae]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Download         Length: 270 bp

>T144855 NZ_CP062243:4343932-4344105 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10088.58 Da        Isoelectric Point: 6.0728

>AT144855 WP_001258569.1 NZ_CP046741:c540105-539827 [Vibrio cholerae]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Download         Length: 279 bp

>AT144855 NZ_CP062243:4343608-4343904 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A151JGK8

Antitoxin

Source ID Structure
AlphaFold DB A0A151KTP9

References