Detailed information of TA system TA144854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 538467..538965
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GPW86_RS02750
• Protein ID: WP_000589156.1
• Coordinates: 538467..538733 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D7HCY3
• Locus tag: GPW86_RS02755
• Protein ID: WP_000643600.1
• Coordinates: 538720..538965 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS02705	533739..534527	+	789	WP_071186407.1	hypothetical protein	-
GPW86_RS02715	534680..535075	+	396	WP_000870864.1	DUF3465 domain-containing protein	-
GPW86_RS02720	535222..535671	+	450	WP_057556343.1	GyrI-like domain-containing protein	-
GPW86_RS02725	535658..535786	+	129	WP_080390558.1	DUF3265 domain-containing protein	-
GPW86_RS02730	535864..536379	+	516	WP_000284464.1	hypothetical protein	-
GPW86_RS02735	537022..537273	+	252	WP_123011643.1	hypothetical protein	-
GPW86_RS02740	537270..537485	+	216	WP_123011644.1	hypothetical protein	-
GPW86_RS02745	537634..538341	+	708	WP_001893912.1	HAD family hydrolase	-
GPW86_RS02750	538467..538733	+	267	WP_000589156.1	BrnT family toxin	Toxin
GPW86_RS02755	538720..538965	+	246	WP_000643600.1	hypothetical protein	Antitoxin
GPW86_RS02760	539143..539427	+	285	WP_000048765.1	antibiotic biosynthesis monooxygenase	-
GPW86_RS02765	539565..539834	-	270	WP_000277238.1	type II toxin-antitoxin system YafQ family toxin	-
GPW86_RS02770	539827..540105	-	279	WP_001258569.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GPW86_RS02775	540588..540638	+	51	Protein_531	DUF645 family protein	-
GPW86_RS02785	540921..541208	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPW86_RS02790	541219..541536	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
GPW86_RS19365	541837..542035	+	199	Protein_534	DUF645 family protein	-
GPW86_RS02805	542377..542556	+	180	WP_032470611.1	DUF645 family protein	-
GPW86_RS02810	542798..543121	+	324	WP_080476600.1	DUF3709 domain-containing protein	-
GPW86_RS02815	543440..543955	+	516	WP_000284464.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	444653..571022	126369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T144854 WP_000589156.1 NZ_CP046741:538467-538733 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T144854 NZ_CP062243:c3694654-3694547 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9446.71 Da
• Isoelectric Point: 6.4832

>AT144854 WP_000643600.1 NZ_CP046741:538720-538965 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVVDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT144854 NZ_CP062243:3694703-3694769 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: D7HCY3