Detailed information of TA system TA144850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 450132..450691
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HHK2
• Locus tag: GPW86_RS01920
• Protein ID: WP_000578471.1
• Coordinates: 450132..450410 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GPW86_RS01925
• Protein ID: WP_076008603.1
• Coordinates: 450407..450691 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS01885	446193..446303	+	111	WP_072599312.1	DUF3265 domain-containing protein	-
GPW86_RS01890	446342..446650	-	309	WP_198135908.1	thiamine-binding protein	-
GPW86_RS01895	446928..447281	+	354	WP_072609772.1	hypothetical protein	-
GPW86_RS01900	447278..447388	+	111	WP_123011821.1	DUF3265 domain-containing protein	-
GPW86_RS01905	447420..448199	+	780	WP_123011587.1	alpha/beta hydrolase	-
GPW86_RS01910	448341..448934	+	594	WP_114774756.1	hypothetical protein	-
GPW86_RS01915	449095..449992	+	898	Protein_375	LD-carboxypeptidase	-
GPW86_RS01920	450132..450410	-	279	WP_000578471.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
GPW86_RS01925	450407..450691	-	285	WP_076008603.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPW86_RS01930	450917..452134	+	1218	WP_103156255.1	DUF4145 domain-containing protein	-
GPW86_RS01935	452618..452740	+	123	WP_123011822.1	DUF3265 domain-containing protein	-
GPW86_RS01940	452756..453325	+	570	WP_123011588.1	hypothetical protein	-
GPW86_RS01945	453347..453439	+	93	WP_078555132.1	DUF3265 domain-containing protein	-
GPW86_RS01955	453895..454767	+	873	WP_000621922.1	hypothetical protein	-
GPW86_RS01960	455065..455190	+	126	WP_052147640.1	DUF645 family protein	-
GPW86_RS01965	455206..455244	+	39	WP_108244118.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	444653..571022	126369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10838.56 Da
• Isoelectric Point: 4.6794

>T144850 WP_000578471.1 NZ_CP046741:c450410-450132 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T144850 NZ_CP062243:c3034182-3033775 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10929.26 Da
• Isoelectric Point: 8.0793

>AT144850 WP_076008603.1 NZ_CP046741:c450691-450407 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKARIRNRGHA

Nucleotide

• Length: 285 bp

>AT144850 NZ_CP062243:c3034429-3034163 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A271VKS2