Detailed information of TA system TA144848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 159061..160736
• Replicon: chromosome
• Accession: NZ_CP046740
• Organism: Vibrio cholerae strain 3566-06

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: GPW70_RS05705
• Protein ID: WP_032473081.1
• Coordinates: 159525..160736 (+)
• Length: 404 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0X8LPP8
• Locus tag: GPW70_RS05700
• Protein ID: WP_000649312.1
• Coordinates: 159061..159528 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW70_RS05670	154260..154481	-	222	WP_005378664.1	AlpA family transcriptional regulator	-
GPW70_RS05675	154559..155548	+	990	WP_032473084.1	50S ribosome-binding GTPase	-
GPW70_RS05680	155659..156105	+	447	WP_032473083.1	hypothetical protein	-
GPW70_RS05685	156146..156790	-	645	WP_032473082.1	inovirus Gp2 family protein	-
GPW70_RS05690	156957..158081	-	1125	WP_031779007.1	hypothetical protein	-
GPW70_RS05695	158136..158807	+	672	WP_001888975.1	hypothetical protein	-
GPW70_RS05700	159061..159528	+	468	WP_000649312.1	DUF4065 domain-containing protein	Antitoxin
GPW70_RS05705	159525..160736	+	1212	WP_032473081.1	RelA/SpoT domain-containing protein	Toxin
GPW70_RS05710	160982..162091	-	1110	WP_001888924.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
GPW70_RS05715	162489..164321	+	1833	WP_019829931.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (86-226)

Antitoxin

• Antitoxin SocA-like, Panacea domain (32-123)

Sequences

Toxin

Protein

• Length: 404 a.a.
• Molecular weight: 45807.63 Da
• Isoelectric Point: 5.2762

>T144848 WP_032473081.1 NZ_CP046740:159525-160736 [Vibrio cholerae]
VSDKEKRVVSFQIDDTPTAEQASKNFQDDYELCFQDFLERTVDESAAEDFSYSNNGVKKAGRRLRKKEGDLKSATATIQQ
FRAAHEKPLNTIAYLVGRCCRELGIDVKPVTRLKRLETIVDKLQRKSLDGETTNATCVTNMNDIGGCRAIFPDLESLEKA
KERLQVTVEKVGRIKIKDIDDYIANPKPNDCGYRSLHVIYRYDHASGKSFNIEAQLRTRLQHLWATTVEIVDILEGTKIK
THSHSPDEDKEKLQVQWEELLSIMSQYIADAEGAIELSNDNRTQFSNRLIELNREINALSRLRSFKILSEKVEGCRDGSI
EHVLLVIDETSLEMIFSEEFENYTQAISVYNAAEKMTRSHARLNTLLISTKNMGQLSEAYPNYLGDCASFITLLEDAMQS
GQN

Nucleotide

• Length: 1212 bp

>T144848 NZ_CP062243:2770812-2771141 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 7.5659

>AT144848 WP_000649312.1 NZ_CP046740:159061-159528 [Vibrio cholerae]
MKAYHTYSAVDVAWSLLKHAKEQGKCFSNLQLQKLTYVCHGLSLAHFQRPLVVDDVFAWKFGPVVPSVYFRFKSYGSNVI
TEQCDVVLDHESESIVKDVVSQLGHLTGPQLVDLTHREGSPWHQVWDGTHQKVIPDHLIQAHYTQIKQSGRTASL

Nucleotide

• Length: 468 bp

>AT144848 NZ_CP062243:2770474-2770791 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0X8LPP8