Detailed information of TA system TA144846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 66647..67175
• Replicon: chromosome
• Accession: NZ_CP046739
• Organism: Vibrio cholerae strain 3566-06

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: GPW70_RS00605
• Protein ID: WP_000221352.1
• Coordinates: 66885..67175 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: GPW70_RS00600
• Protein ID: WP_000213183.1
• Coordinates: 66647..66895 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW70_RS00565	61910..62053	-	144	WP_080360960.1	DUF645 family protein	-
GPW70_RS00570	62371..62778	-	408	WP_032473180.1	DUF1398 family protein	-
GPW70_RS00575	62935..63342	-	408	WP_032473179.1	hypothetical protein	-
GPW70_RS00580	63490..63903	-	414	WP_001887498.1	VOC family protein	-
GPW70_RS00585	64099..64947	-	849	WP_032473178.1	nucleotide-binding protein	-
GPW70_RS00590	65143..65724	-	582	WP_032473177.1	HD domain-containing protein	-
GPW70_RS00595	65892..66440	-	549	WP_032473176.1	hypothetical protein	-
GPW70_RS00600	66647..66895	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW70_RS00605	66885..67175	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW70_RS00610	67360..67398	-	39	WP_108244114.1	hypothetical protein	-
GPW70_RS00615	67439..67594	-	156	Protein_114	DUF645 family protein	-
GPW70_RS00620	67850..68212	-	363	WP_161779867.1	DUF4144 domain-containing protein	-
GPW70_RS00625	68303..68737	-	435	WP_043881279.1	GNAT family N-acetyltransferase	-
GPW70_RS00630	68878..69678	-	801	WP_001887496.1	PhzF family phenazine biosynthesis protein	-
GPW70_RS00635	70112..70627	+	516	WP_158106810.1	hypothetical protein	-
GPW70_RS00640	70761..71369	-	609	WP_032473184.1	hypothetical protein	-
GPW70_RS00645	71575..71741	-	167	Protein_120	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA / zot / ace / zot / ace / cqsA	39..1050830	1050791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T144846 WP_000221352.1 NZ_CP046739:66885-67175 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T144846 NZ_CP062243:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT144846 WP_000213183.1 NZ_CP046739:66647-66895 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT144846 NZ_CP062243:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8