Detailed information of TA system TA144843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 18042..18808
• Replicon: chromosome
• Accession: NZ_CP046739
• Organism: Vibrio cholerae strain 3566-06

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: GPW70_RS00160
• Protein ID: WP_000982260.1
• Coordinates: 18311..18808 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GPW70_RS00155
• Protein ID: WP_019829878.1
• Coordinates: 18042..18314 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW70_RS00110	13293..13673	-	381	WP_032473190.1	hypothetical protein	-
GPW70_RS00130	14329..14556	-	228	WP_080286755.1	DUF1289 domain-containing protein	-
GPW70_RS18935	14505..14618	-	114	WP_080286756.1	acetyltransferase	-
GPW70_RS00135	14675..15253	-	579	WP_032473189.1	DJ-1/PfpI family protein	-
GPW70_RS00140	15407..16045	-	639	WP_019829928.1	hypothetical protein	-
GPW70_RS00145	16180..16677	-	498	WP_005526565.1	GNAT family N-acetyltransferase	-
GPW70_RS00150	16882..17052	-	171	WP_000483444.1	DUF645 family protein	-
GPW70_RS00155	18042..18314	+	273	WP_019829878.1	DUF1778 domain-containing protein	Antitoxin
GPW70_RS00160	18311..18808	+	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
GPW70_RS00165	18943..19902	-	960	WP_001887517.1	restriction endonuclease	-
GPW70_RS00170	20489..20944	-	456	WP_001245329.1	GNAT family N-acetyltransferase	-
GPW70_RS00175	21071..21502	-	432	WP_044128141.1	hypothetical protein	-
GPW70_RS00180	21643..22416	-	774	WP_139284613.1	hypothetical protein	-
GPW70_RS00185	22550..23034	-	485	Protein_32	DUF1993 family protein	-
GPW70_RS00190	23192..23581	-	390	WP_000491261.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA / zot / ace / zot / ace / cqsA	39..1050830	1050791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T144843 WP_000982260.1 NZ_CP046739:18311-18808 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T144843 NZ_CP062243:c1917464-1917361 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9713.24 Da
• Isoelectric Point: 6.2988

>AT144843 WP_019829878.1 NZ_CP046739:18042-18314 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQTDAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT144843 NZ_CP062243:1917323-1917594 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1