Detailed information of TA system TA144842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 10929..11457
• Replicon: chromosome
• Accession: NZ_CP046739
• Organism: Vibrio cholerae strain 3566-06

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: GPW70_RS00090
• Protein ID: WP_000221352.1
• Coordinates: 11167..11457 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0Q0JPP0
• Locus tag: GPW70_RS00085
• Protein ID: WP_019829909.1
• Coordinates: 10929..11177 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW70_RS00050	6247..6699	-	453	Protein_8	hypothetical protein	-
GPW70_RS00055	6791..8113	+	1323	WP_001886535.1	IS4 family transposase	-
GPW70_RS00060	8127..8537	-	411	Protein_10	hypothetical protein	-
GPW70_RS00065	8683..9330	-	648	WP_032473193.1	glutathione S-transferase family protein	-
GPW70_RS00070	9484..9900	-	417	WP_019829470.1	hypothetical protein	-
GPW70_RS00080	10065..10718	-	654	WP_019829910.1	peptidoglycan DD-metalloendopeptidase family protein	-
GPW70_RS00085	10929..11177	+	249	WP_019829909.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW70_RS00090	11167..11457	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW70_RS00095	11585..12106	-	522	WP_019829942.1	GrpB family protein	-
GPW70_RS00100	12302..12484	-	183	WP_019829944.1	DUF645 family protein	-
GPW70_RS00105	12832..12975	-	144	WP_080360960.1	DUF645 family protein	-
GPW70_RS00110	13293..13673	-	381	WP_032473190.1	hypothetical protein	-
GPW70_RS00130	14329..14556	-	228	WP_080286755.1	DUF1289 domain-containing protein	-
GPW70_RS18935	14505..14618	-	114	WP_080286756.1	acetyltransferase	-
GPW70_RS00135	14675..15253	-	579	WP_032473189.1	DJ-1/PfpI family protein	-
GPW70_RS00140	15407..16045	-	639	WP_019829928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	ugd / htpB / hlyA / hcp-2 / vgrG-2 / vipA/mglA / vipB/mglB / VCA0109 / vasA / vasB / vasC / vasD / vasE / vasF / clpB/vasG / vasH / vasI / vasJ / icmF/vasK / vasL / vgrG-3 / hlyA / zot / ace / zot / ace / cqsA	39..1050830	1050791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T144842 WP_000221352.1 NZ_CP046739:11167-11457 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T144842 NZ_CP062243:c1639890-1639603 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8967.31 Da
• Isoelectric Point: 4.1079

>AT144842 WP_019829909.1 NZ_CP046739:10929-11177 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAITKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT144842 NZ_CP062243:c1640129-1639890 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A0Q0JPP0