Detailed information of TA system TA144840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1046010..1046548
• Replicon: chromosome
• Accession: NZ_CP046738
• Organism: Vibrio cholerae strain 2015V-1126

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: GPW74_RS18075
• Protein ID: WP_000802136.1
• Coordinates: 1046249..1046548 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: GPW74_RS18070
• Protein ID: WP_001107720.1
• Coordinates: 1046010..1046252 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW74_RS18790	1041076..1041165	-	90	WP_001905720.1	DUF645 family protein	-
GPW74_RS18030	1041523..1041693	-	171	WP_001928536.1	DUF645 family protein	-
GPW74_RS18035	1042130..1042366	-	237	WP_123012100.1	DUF3709 domain-containing protein	-
GPW74_RS18045	1043038..1043427	-	390	WP_184478429.1	VOC family protein	-
GPW74_RS18050	1043642..1044265	-	624	WP_198301107.1	hypothetical protein	-
GPW74_RS18060	1044822..1045238	-	417	WP_000759008.1	DUF1311 domain-containing protein	-
GPW74_RS18065	1045386..1045781	-	396	WP_000870861.1	DUF3465 domain-containing protein	-
GPW74_RS18070	1046010..1046252	+	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GPW74_RS18075	1046249..1046548	+	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW74_RS18080	1046726..1046908	-	183	WP_000923184.1	DUF645 family protein	-
GPW74_RS18085	1047206..1047601	-	396	WP_046121682.1	DUF3465 domain-containing protein	-
GPW74_RS18795	1047754..1048335	-	582	WP_114736614.1	hypothetical protein	-
GPW74_RS18095	1048333..1048398	-	66	Protein_952	nitrate reductase	-
GPW74_RS18800	1048525..1048563	-	39	WP_082798268.1	hypothetical protein	-
GPW74_RS18805	1048617..1048760	-	144	Protein_954	DUF645 family protein	-
GPW74_RS18105	1049003..1050265	-	1263	WP_114743463.1	hypothetical protein	-
GPW74_RS18115	1050415..1051281	-	867	WP_123012145.1	PSE family carbenicillin-hydrolyzing class A beta-lactamase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	949375..1072270	122895
	-	inside	Genomic island	blaCARB-7	-	949582..1075528	125946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T144840 WP_000802136.1 NZ_CP046738:1046249-1046548 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T144840 NZ_CP062243:1503543-1503719 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT144840 WP_001107720.1 NZ_CP046738:1046010-1046252 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT144840 NZ_CP062243:1503765-1504181 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• AlphaFold DB: A0A271VK51