Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1030350..1030848 Replicon chromosome
Accession NZ_CP046738
Organism Vibrio cholerae strain 2015V-1126

Toxin (Protein)


Gene name - Uniprot ID A0A151JG87
Locus tag GPW74_RS17935 Protein ID WP_000589153.1
Coordinates 1030582..1030848 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A151JFU0
Locus tag GPW74_RS17930 Protein ID WP_000643599.1
Coordinates 1030350..1030595 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW74_RS17890 1025563..1025773 - 211 Protein_914 DUF3709 domain-containing protein -
GPW74_RS17895 1025809..1025970 - 162 WP_113625331.1 DUF3709 domain-containing protein -
GPW74_RS17900 1026193..1026426 - 234 WP_076008639.1 DUF3709 domain-containing protein -
GPW74_RS17910 1026758..1026937 - 180 WP_123012090.1 DUF645 family protein -
GPW74_RS17915 1027308..1027568 + 261 WP_080551836.1 hypothetical protein -
GPW74_RS18780 1027756..1028181 - 426 WP_184478425.1 hypothetical protein -
GPW74_RS17920 1028183..1029037 - 855 WP_148038999.1 GAF domain-containing protein -
GPW74_RS17925 1029840..1030058 - 219 WP_184478427.1 DUF3709 domain-containing protein -
GPW74_RS17930 1030350..1030595 - 246 WP_000643599.1 hypothetical protein Antitoxin
GPW74_RS17935 1030582..1030848 - 267 WP_000589153.1 BrnT family toxin Toxin
GPW74_RS17940 1031024..1031491 - 468 WP_123012094.1 OsmC family protein -
GPW74_RS17945 1031523..1031687 - 165 WP_123012095.1 DUF3265 domain-containing protein -
GPW74_RS17950 1031647..1032489 - 843 WP_095464235.1 nucleotide-binding protein -
GPW74_RS17955 1032707..1032928 - 222 WP_088129314.1 hypothetical protein -
GPW74_RS17960 1033099..1033947 - 849 WP_032473178.1 nucleotide-binding protein -
GPW74_RS17970 1034636..1034818 - 183 Protein_929 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 949375..1072270 122895
- inside Genomic island blaCARB-7 - 949582..1075528 125946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10062.32 Da        Isoelectric Point: 7.3171

>T144839 WP_000589153.1 NZ_CP046738:c1030848-1030582 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGANIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T144839 NZ_CP062243:c1408184-1407990 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9504.80 Da        Isoelectric Point: 6.2241

>AT144839 WP_000643599.1 NZ_CP046738:c1030595-1030350 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMYKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT144839 NZ_CP062243:1407813-1407991 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A151JG87

Antitoxin

Source ID Structure
AlphaFold DB A0A151JFU0

References