Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1007508..1008006 Replicon chromosome
Accession NZ_CP046735
Organism Vibrio cholerae strain 3528-08

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag GPW66_RS04510 Protein ID WP_000589156.1
Coordinates 1007740..1008006 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag GPW66_RS04505 Protein ID WP_000643598.1
Coordinates 1007508..1007753 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW66_RS04465 1003186..1003670 - 485 Protein_867 DUF1993 family protein -
GPW66_RS04470 1003828..1004217 - 390 WP_000491261.1 VOC family protein -
GPW66_RS04475 1004421..1004873 - 453 WP_032473166.1 GNAT family N-acetyltransferase -
GPW66_RS04480 1005025..1005465 - 441 WP_032473167.1 DUF1311 domain-containing protein -
GPW66_RS04485 1005727..1005987 - 261 WP_032473168.1 DUF3709 domain-containing protein -
GPW66_RS04490 1006005..1006199 - 195 WP_154081050.1 DUF3709 domain-containing protein -
GPW66_RS04495 1006256..1006792 - 537 WP_019829641.1 nucleotidyltransferase family protein -
GPW66_RS04500 1006948..1007364 - 417 WP_019830302.1 DUF1311 domain-containing protein -
GPW66_RS04505 1007508..1007753 - 246 WP_000643598.1 hypothetical protein Antitoxin
GPW66_RS04510 1007740..1008006 - 267 WP_000589156.1 BrnT family toxin Toxin
GPW66_RS04515 1008182..1008733 - 552 WP_019829924.1 VRR-NUC domain-containing protein -
GPW66_RS04525 1008897..1009448 - 552 WP_019829627.1 sugar O-acetyltransferase -
GPW66_RS04530 1010057..1010569 - 513 WP_158106265.1 hypothetical protein -
GPW66_RS04535 1010844..1011326 - 483 WP_142542431.1 GNAT family N-acetyltransferase -
GPW66_RS04540 1011474..1012070 - 597 WP_158106266.1 DJ-1/PfpI family protein -
GPW66_RS04545 1012265..1012303 - 39 WP_108244118.1 hypothetical protein -
GPW66_RS04550 1012319..1012440 - 122 Protein_883 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 990327..1025301 34974
- inside Integron - hlyA / vgrG-3 / vasL / icmF/vasK / vasJ / vasI / vasH / clpB/vasG / vasF / vasE / vasD / vasC / vasB / vasA / VCA0109 / vipB/mglB / vipA/mglA / vgrG-2 / hcp-2 / hlyA / htpB / ugd / cqsA 2..1025486 1025484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T144827 WP_000589156.1 NZ_CP046735:c1008006-1007740 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T144827 NZ_CP062242:c3693447-3693340 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT144827 WP_000643598.1 NZ_CP046735:c1007753-1007508 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT144827 NZ_CP062242:3693496-3693562 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References