Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/ParE-RHH |
| Location | 12443..12981 | Replicon | chromosome |
| Accession | NZ_CP046735 | ||
| Organism | Vibrio cholerae strain 3528-08 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | Q9KMJ0 |
| Locus tag | GPW66_RS00125 | Protein ID | WP_000802136.1 |
| Coordinates | 12682..12981 (+) | Length | 100 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | P58093 |
| Locus tag | GPW66_RS00120 | Protein ID | WP_001107719.1 |
| Coordinates | 12443..12685 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GPW66_RS00080 | 7884..8249 | - | 366 | WP_019830314.1 | hypothetical protein | - |
| GPW66_RS00085 | 8433..8615 | - | 183 | WP_032473221.1 | DUF645 family protein | - |
| GPW66_RS00090 | 8912..9400 | - | 489 | WP_000110032.1 | GNAT family N-acetyltransferase | - |
| GPW66_RS00095 | 9547..9981 | - | 435 | WP_000256034.1 | GNAT family N-acetyltransferase | - |
| GPW66_RS00105 | 10118..10504 | - | 387 | WP_043881269.1 | hypothetical protein | - |
| GPW66_RS00110 | 10694..11593 | - | 900 | WP_001887493.1 | hypothetical protein | - |
| GPW66_RS00115 | 11738..12226 | - | 489 | WP_000424237.1 | hypothetical protein | - |
| GPW66_RS00120 | 12443..12685 | + | 243 | WP_001107719.1 | type II toxin-antitoxin system ParD family antitoxin | Antitoxin |
| GPW66_RS00125 | 12682..12981 | + | 300 | WP_000802136.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| GPW66_RS00135 | 13202..13579 | + | 378 | WP_000734783.1 | PEGA domain-containing protein | - |
| GPW66_RS00140 | 13744..14358 | - | 615 | WP_032473157.1 | hypothetical protein | - |
| GPW66_RS00145 | 14513..14971 | - | 459 | WP_032473158.1 | GNAT family N-acetyltransferase | - |
| GPW66_RS00155 | 15122..15493 | - | 372 | WP_032473159.1 | nucleotide pyrophosphohydrolase | - |
| GPW66_RS00160 | 15640..16464 | - | 825 | WP_148508194.1 | hypothetical protein | - |
| GPW66_RS00165 | 16612..17133 | - | 522 | WP_158106111.1 | cysteine hydrolase | - |
| GPW66_RS00170 | 17280..17744 | - | 465 | WP_032473198.1 | ASCH domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integron | - | hlyA / vgrG-3 / vasL / icmF/vasK / vasJ / vasI / vasH / clpB/vasG / vasF / vasE / vasD / vasC / vasB / vasA / VCA0109 / vipB/mglB / vipA/mglA / vgrG-2 / hcp-2 / hlyA / htpB / ugd / cqsA | 2..1025486 | 1025484 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 100 a.a. Molecular weight: 11589.29 Da Isoelectric Point: 9.9232
>T144825 WP_000802136.1 NZ_CP046735:12682-12981 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA
Download Length: 300 bp
>T144825 NZ_CP062242:c3227814-3227500 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 81 a.a. Molecular weight: 8964.84 Da Isoelectric Point: 4.1677
>AT144825 WP_001107719.1 NZ_CP046735:12443-12685 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR
Download Length: 243 bp
>AT144825 NZ_CP062242:c3227503-3227087 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 7R5A |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 7R5A | |
| PDB | 7B22 | |
| AlphaFold DB | A0A151JFU4 |