Detailed information of TA system TA144823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1188926..1189536
• Replicon: plasmid pLLRS-1
• Accession: NZ_CP046730
• Organism: Ralstonia syzygii strain LLRS-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GO998_RS21595
• Protein ID: WP_075465902.1
• Coordinates: 1188926..1189234 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GO998_RS21600
• Protein ID: WP_013209163.1
• Coordinates: 1189231..1189536 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO998_RS21570 (GO998_21550)	1184547..1184981	-	435	WP_013209169.1	VOC family protein	-
GO998_RS21575 (GO998_21555)	1185243..1185839	-	597	WP_075465909.1	GNAT family N-acetyltransferase	-
GO998_RS21580 (GO998_21560)	1185971..1186525	+	555	WP_075465907.1	helix-turn-helix domain-containing protein	-
GO998_RS21585 (GO998_21565)	1186699..1187514	+	816	WP_075465905.1	GntR family transcriptional regulator	-
GO998_RS21590 (GO998_21570)	1187642..1188841	+	1200	WP_211905177.1	MFS transporter	-
GO998_RS21595 (GO998_21575)	1188926..1189234	+	309	WP_075465902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GO998_RS21600 (GO998_21580)	1189231..1189536	+	306	WP_013209163.1	putative addiction module antidote protein	Antitoxin
GO998_RS21605 (GO998_21585)	1189747..1191060	+	1314	WP_211905178.1	MFS family transporter	-
GO998_RS21610 (GO998_21590)	1191165..1191860	-	696	WP_230426055.1	hypothetical protein	-
GO998_RS21615 (GO998_21595)	1192000..1192187	-	188	Protein_891	transposase	-
GO998_RS21620 (GO998_21600)	1192521..1192805	+	285	WP_075465896.1	hypothetical protein	-
GO998_RS21625 (GO998_21605)	1192938..1193219	+	282	WP_211905179.1	hypothetical protein	-
GO998_RS21630 (GO998_21610)	1193477..1193869	-	393	WP_013209159.1	RidA family protein	-
GO998_RS21635 (GO998_21615)	1193907..1194317	-	411	WP_075465861.1	thioesterase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / fliI / fliG / fliM / flgG / flgC / cyaB / cheY / motA / cheW / cheB / cheY / adeG	1..2046405	2046405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11795.54 Da
• Isoelectric Point: 8.5237

>T144823 WP_075465902.1 NZ_CP046730:1188926-1189234 [Ralstonia syzygii]
MLTIRTTEIFDDWFCSLRDKAAQRRVQVRIDRLQMGNPGDVKAVREGIREMRIDHGPGYRVYFSQRGVVLIVLLCGGDKS
TQEADVRRAIDLSRRLDVDNME

Nucleotide

• Length: 309 bp

>T144823 NZ_CP062242:c3034182-3033775 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11009.67 Da
• Isoelectric Point: 6.7174

>AT144823 WP_013209163.1 NZ_CP046730:1189231-1189536 [Ralstonia syzygii]
MMMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARARGMTQIARETGMTREGLYKALSEDGNP
SFATVLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT144823 NZ_CP062242:c3034429-3034163 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA