Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3703468..3704142 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag GP477_RS17430 Protein ID WP_003417282.1
Coordinates 3703468..3703896 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag GP477_RS17435 Protein ID WP_003417286.1
Coordinates 3703900..3704142 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS17405 3699290..3699691 - 402 WP_003417264.1 cytidine deaminase -
GP477_RS17410 3699832..3700266 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
GP477_RS17415 3700263..3700697 + 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
GP477_RS17420 3700826..3702598 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
GP477_RS17425 3702598..3703389 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
GP477_RS17430 3703468..3703896 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
GP477_RS17435 3703900..3704142 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
GP477_RS17440 3704264..3704878 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
GP477_RS17445 3704875..3705540 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
GP477_RS17450 3705541..3706074 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
GP477_RS17455 3706071..3706445 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
GP477_RS17460 3706542..3707606 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T144817 WP_003417282.1 NZ_CP046728:c3703896-3703468 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T144817 NZ_CP062242:2071050-2071424 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT144817 WP_003417286.1 NZ_CP046728:c3704142-3703900 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT144817 NZ_CP062242:2070593-2070961 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References