Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3170247..3170934 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag GP477_RS15010 Protein ID WP_003414624.1
Coordinates 3170491..3170934 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag GP477_RS15005 Protein ID WP_003414620.1
Coordinates 3170247..3170504 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS14985 3165567..3166421 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
GP477_RS14990 3166477..3167640 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
GP477_RS14995 3167657..3168871 - 1215 WP_003414610.1 zinc metalloprotease Rip -
GP477_RS15000 3168879..3170120 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
GP477_RS15005 3170247..3170504 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
GP477_RS15010 3170491..3170934 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
GP477_RS15015 3171014..3171676 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
GP477_RS15020 3171772..3171963 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GP477_RS15025 3171956..3174043 + 2088 WP_003414635.1 cytochrome c biogenesis protein DipZ -
GP477_RS15030 3174139..3174720 + 582 WP_003414644.1 fasciclin domain-containing protein -
GP477_RS15035 3174772..3175086 + 315 WP_003899522.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T144815 WP_003414624.1 NZ_CP046728:3170491-3170934 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T144815 NZ_CP062242:c1639890-1639603 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT144815 WP_003414620.1 NZ_CP046728:3170247-3170504 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT144815 NZ_CP062242:c1640129-1639890 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References