Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3125187..3125791 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag GP477_RS14785 Protein ID WP_003414492.1
Coordinates 3125187..3125579 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag GP477_RS14790 Protein ID WP_003414495.1
Coordinates 3125576..3125791 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS14755 3120337..3121059 - 723 WP_003912014.1 CRISPR-associated endoribonuclease Cas6 -
GP477_RS14760 3121459..3122004 - 546 WP_014584866.1 DUF1802 family protein -
GP477_RS14765 3122276..3123160 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GP477_RS14770 3123163..3124050 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
GP477_RS14775 3124355..3124900 - 546 WP_003910939.1 DUF1802 family protein -
GP477_RS14780 3124897..3125166 - 270 WP_003912856.1 DUF2277 family protein -
GP477_RS14785 3125187..3125579 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
GP477_RS14790 3125576..3125791 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GP477_RS14795 3125838..3126587 + 750 WP_003902358.1 enoyl-CoA hydratase -
GP477_RS14800 3126666..3127748 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
GP477_RS14805 3127741..3129051 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
GP477_RS14810 3129054..3129881 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
GP477_RS14815 3129878..3130789 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T144813 WP_003414492.1 NZ_CP046728:c3125579-3125187 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T144813 NZ_CP062242:1503543-1503719 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT144813 WP_003414495.1 NZ_CP046728:c3125791-3125576 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT144813 NZ_CP062242:1503765-1504181 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References