Detailed information of TA system TA144812

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 3122276..3124050
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: GP477_RS14765
• Protein ID: WP_003414409.1
• Coordinates: 3122276..3123160 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: GP477_RS14770
• Protein ID: WP_003414414.1
• Coordinates: 3123163..3124050 (-)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS14745	3117531..3117905	-	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-
GP477_RS14750	3117902..3120340	-	2439	WP_003900580.1	type III-A CRISPR-associated protein Cas10/Csm1	-
GP477_RS14755	3120337..3121059	-	723	WP_003912014.1	CRISPR-associated endoribonuclease Cas6	-
GP477_RS14760	3121459..3122004	-	546	WP_014584866.1	DUF1802 family protein	-
GP477_RS14765	3122276..3123160	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GP477_RS14770	3123163..3124050	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GP477_RS14775	3124355..3124900	-	546	WP_003910939.1	DUF1802 family protein	-
GP477_RS14780	3124897..3125166	-	270	WP_003912856.1	DUF2277 family protein	-
GP477_RS14785	3125187..3125579	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
GP477_RS14790	3125576..3125791	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GP477_RS14795	3125838..3126587	+	750	WP_003902358.1	enoyl-CoA hydratase	-
GP477_RS14800	3126666..3127748	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T144812 WP_003414409.1 NZ_CP046728:c3123160-3122276 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

>T144812 NZ_CP062242:c1408184-1407990 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT144812 WP_003414414.1 NZ_CP046728:c3124050-3123163 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

>AT144812 NZ_CP062242:1407813-1407991 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9