Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3104989..3105559 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag GP477_RS14660 Protein ID WP_003414166.1
Coordinates 3104989..3105345 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag GP477_RS14665 Protein ID WP_003901465.1
Coordinates 3105329..3105559 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS14640 3100441..3102129 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
GP477_RS14645 3102133..3102459 - 327 WP_003414157.1 hypothetical protein -
GP477_RS14650 3102590..3103219 + 630 WP_003414158.1 DUF3558 family protein -
GP477_RS14655 3103238..3104887 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
GP477_RS14660 3104989..3105345 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
GP477_RS14665 3105329..3105559 - 231 WP_003901465.1 antitoxin MazE Antitoxin
GP477_RS14670 3105602..3106645 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
GP477_RS14675 3106836..3107111 + 276 WP_003911993.1 DUF1778 domain-containing protein -
GP477_RS14680 3107287..3107541 - 255 WP_003917684.1 hypothetical protein -
GP477_RS14685 3107689..3108093 + 405 WP_003414181.1 hypothetical protein -
GP477_RS14690 3108090..3108281 + 192 WP_003414184.1 hypothetical protein -
GP477_RS14695 3108480..3109634 + 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
GP477_RS14700 3109868..3110125 + 258 WP_003899489.1 hypothetical protein -
GP477_RS14705 3110230..3110541 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T144811 WP_003414166.1 NZ_CP046728:c3105345-3104989 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T144811 NZ_CP062242:c1265801-1265694 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT144811 WP_003901465.1 NZ_CP046728:c3105559-3105329 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT144811 NZ_CP062242:1265848-1265914 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References