Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3064991..3065670 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag GP477_RS14440 Protein ID WP_003414059.1
Coordinates 3064991..3065407 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag GP477_RS14445 Protein ID WP_003414061.1
Coordinates 3065404..3065670 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS14420 3061043..3061945 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
GP477_RS14425 3062014..3062766 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
GP477_RS14430 3063010..3063285 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
GP477_RS14435 3063282..3064904 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
GP477_RS14440 3064991..3065407 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
GP477_RS14445 3065404..3065670 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GP477_RS14450 3065696..3066091 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS14455 3066088..3066357 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
GP477_RS14460 3066367..3067461 - 1095 WP_003414068.1 restriction endonuclease subunit S -
GP477_RS14465 3067458..3067877 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
GP477_RS14470 3067951..3068430 - 480 WP_003414073.1 dihydrofolate reductase -
GP477_RS14475 3068501..3069301 - 801 WP_003911953.1 thymidylate synthase -
GP477_RS14480 3069457..3070194 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T144809 WP_003414059.1 NZ_CP046728:c3065407-3064991 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T144809 NZ_CP062242:c1264731-1264624 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT144809 WP_003414061.1 NZ_CP046728:c3065670-3065404 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT144809 NZ_CP062242:1264779-1264845 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References