Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2866037..2866674 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag GP477_RS13360 Protein ID WP_003413180.1
Coordinates 2866037..2866432 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag GP477_RS13365 Protein ID WP_003413183.1
Coordinates 2866429..2866674 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS13320 2861441..2862652 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
GP477_RS13325 2862779..2863438 + 660 WP_003902296.1 hypothetical protein -
GP477_RS13330 2863435..2864097 + 663 WP_003905878.1 hypothetical protein -
GP477_RS13335 2864094..2864371 + 278 Protein_2632 type II toxin-antitoxin system VapB family antitoxin -
GP477_RS13340 2864464..2864877 + 414 WP_003413164.1 PIN domain nuclease -
GP477_RS13345 2864916..2865173 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
GP477_RS13350 2865170..2865547 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS13355 2865563..2865937 - 375 WP_003413177.1 hypothetical protein -
GP477_RS13360 2866037..2866432 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
GP477_RS13365 2866429..2866674 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
GP477_RS13370 2867085..2867504 - 420 WP_003413190.1 A24 family peptidase -
GP477_RS13375 2867516..2868325 - 810 WP_003413193.1 shikimate dehydrogenase -
GP477_RS13380 2868322..2869575 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
GP477_RS13385 2869568..2870080 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T144806 WP_003413180.1 NZ_CP046728:c2866432-2866037 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T144806 NZ_CP062242:248788-249186 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT144806 WP_003413183.1 NZ_CP046728:c2866674-2866429 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT144806 NZ_CP062242:248492-248785 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References