Detailed information of TA system TA144805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2864916..2865547
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: GP477_RS13350
• Protein ID: WP_003413174.1
• Coordinates: 2865170..2865547 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: GP477_RS13345
• Protein ID: WP_003413167.1
• Coordinates: 2864916..2865173 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS13315	2860831..2861145	+	315	WP_009937839.1	hypothetical protein	-
GP477_RS13320	2861441..2862652	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
GP477_RS13325	2862779..2863438	+	660	WP_003902296.1	hypothetical protein	-
GP477_RS13330	2863435..2864097	+	663	WP_003905878.1	hypothetical protein	-
GP477_RS13335	2864094..2864371	+	278	Protein_2632	type II toxin-antitoxin system VapB family antitoxin	-
GP477_RS13340	2864464..2864877	+	414	WP_003413164.1	PIN domain nuclease	-
GP477_RS13345	2864916..2865173	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
GP477_RS13350	2865170..2865547	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
GP477_RS13355	2865563..2865937	-	375	WP_003413177.1	hypothetical protein	-
GP477_RS13360	2866037..2866432	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
GP477_RS13365	2866429..2866674	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
GP477_RS13370	2867085..2867504	-	420	WP_003413190.1	A24 family peptidase	-
GP477_RS13375	2867516..2868325	-	810	WP_003413193.1	shikimate dehydrogenase	-
GP477_RS13380	2868322..2869575	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
GP477_RS13385	2869568..2870080	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T144805 WP_003413174.1 NZ_CP046728:2865170-2865547 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T144805 NZ_CP062242:c242726-242448 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT144805 WP_003413167.1 NZ_CP046728:2864916-2865173 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT144805 NZ_CP062242:c242989-242729 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5