Detailed information of TA system TA144797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2248359..2248985
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: GP477_RS10445
• Protein ID: WP_003410075.1
• Coordinates: 2248587..2248985 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: GP477_RS10440
• Protein ID: WP_003911750.1
• Coordinates: 2248359..2248586 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS10430	2246398..2246742	-	345	WP_003410065.1	ferredoxin family protein	-
GP477_RS10435	2246931..2248256	-	1326	WP_003410070.1	DUF4143 domain-containing protein	-
GP477_RS10440	2248359..2248586	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GP477_RS10445	2248587..2248985	+	399	WP_003410075.1	PIN domain nuclease	Toxin
GP477_RS10450	2249168..2249599	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
GP477_RS10455	2249640..2250134	+	495	WP_003410080.1	DUF1398 domain-containing protein	-
GP477_RS10460	2250571..2250750	-	180	Protein_2067	hypothetical protein	-
GP477_RS10465	2250811..2251458	+	648	WP_071854215.1	transposase	-
GP477_RS10470	2251412..2252002	+	591	WP_003899131.1	IS110 family transposase	-
GP477_RS10475	2252130..2253386	-	1257	WP_003902247.1	HNH endonuclease	-
GP477_RS10480	2253704..2253979	+	276	WP_016330411.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T144797 WP_003410075.1 NZ_CP046728:2248587-2248985 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T144797 NZ_CP062241:c3161903-3161607 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT144797 WP_003911750.1 NZ_CP046728:2248359-2248586 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT144797 NZ_CP062241:c3161605-3161210 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8