Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2215727..2216415 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag GP477_RS10280 Protein ID WP_003409958.1
Coordinates 2215727..2216146 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag GP477_RS10285 Protein ID WP_003409968.1
Coordinates 2216155..2216415 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS10260 2211222..2212070 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
GP477_RS10265 2212033..2213478 - 1446 WP_003899115.1 APC family permease -
GP477_RS10270 2213657..2214343 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
GP477_RS10275 2214534..2215502 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
GP477_RS10280 2215727..2216146 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
GP477_RS10285 2216155..2216415 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GP477_RS10290 2216558..2218234 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
GP477_RS10295 2218222..2218875 - 654 WP_003409976.1 cutinase Cfp21 -
GP477_RS10300 2218990..2219220 + 231 WP_003409980.1 DUF167 domain-containing protein -
GP477_RS10305 2219305..2220216 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
GP477_RS10310 2220325..2220924 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T144795 WP_003409958.1 NZ_CP046728:c2216146-2215727 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T144795 NZ_CP062241:c2904450-2904115 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT144795 WP_003409968.1 NZ_CP046728:c2216415-2216155 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT144795 NZ_CP062241:c2904698-2904450 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References