Detailed information of TA system TA144792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2182278..2182981
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: GP477_RS10050
• Protein ID: WP_003409778.1
• Coordinates: 2182278..2182607 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: GP477_RS10055
• Protein ID: WP_003409780.1
• Coordinates: 2182604..2182981 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS10030	2178661..2179731	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
GP477_RS10035	2179728..2180243	+	516	WP_003409718.1	flavin reductase family protein	-
GP477_RS10040	2180240..2181301	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
GP477_RS10045	2181298..2182068	+	771	WP_003409775.1	SDR family oxidoreductase	-
GP477_RS10050	2182278..2182607	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GP477_RS10055	2182604..2182981	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
GP477_RS10060	2182978..2183568	-	591	WP_003409784.1	SEC-C domain-containing protein	-
GP477_RS10065	2183623..2184987	+	1365	WP_003903691.1	HNH endonuclease	-
GP477_RS10070	2185142..2185594	-	453	WP_003899095.1	lipoprotein	-
GP477_RS10075	2185658..2186059	+	402	WP_003409869.1	hypothetical protein	-
GP477_RS10080	2186052..2186234	-	183	WP_003409870.1	hypothetical protein	-
GP477_RS10085	2186348..2186698	-	351	WP_003409871.1	hypothetical protein	-
GP477_RS10090	2186709..2187611	-	903	WP_003899097.1	hypothetical protein	-
GP477_RS10095	2187632..2187823	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T144792 WP_003409778.1 NZ_CP046728:c2182607-2182278 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T144792 NZ_CP062241:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT144792 WP_003409780.1 NZ_CP046728:c2182981-2182604 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT144792 NZ_CP062241:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8