Detailed information of TA system TA144781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013317..1013936
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: GP477_RS04780
• Protein ID: WP_003404726.1
• Coordinates: 1013502..1013936 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: GP477_RS04775
• Protein ID: WP_003898641.1
• Coordinates: 1013317..1013496 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS04760	1008790..1010370	+	1581	WP_003910977.1	serine hydrolase	-
GP477_RS04765	1010367..1012760	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
GP477_RS04770	1013033..1013191	+	159	WP_003404720.1	hypothetical protein	-
GP477_RS04775	1013317..1013496	+	180	WP_003898641.1	antitoxin	Antitoxin
GP477_RS04780	1013502..1013936	+	435	WP_003404726.1	SRPBCC family protein	Toxin
GP477_RS04785	1014034..1014807	+	774	WP_003404735.1	VOC family protein	-
GP477_RS04790	1014872..1015321	+	450	WP_003404738.1	hypothetical protein	-
GP477_RS04795	1015456..1015686	-	231	WP_003898642.1	hypothetical protein	-
GP477_RS04800	1015853..1017361	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
GP477_RS04805	1017363..1018601	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T144781 WP_003404726.1 NZ_CP046728:1013502-1013936 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T144781 NZ_CP062241:c475764-475546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT144781 WP_003898641.1 NZ_CP046728:1013317-1013496 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT144781 NZ_CP062241:c476164-475790 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5