Detailed information of TA system TA144771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 695537..696183
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: GP477_RS03115
• Protein ID: WP_003403137.1
• Coordinates: 695537..695950 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: GP477_RS03120
• Protein ID: WP_003403139.1
• Coordinates: 695947..696183 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS03095	691620..693170	+	1551	WP_003905349.1	MCE family protein	-
GP477_RS03100	693222..693614	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
GP477_RS03105	693611..693868	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
GP477_RS03110	694051..695286	-	1236	WP_003403128.1	ATP-binding protein	-
GP477_RS03115	695537..695950	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
GP477_RS03120	695947..696183	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GP477_RS03125	696287..696793	-	507	WP_003900973.1	two component system sensor kinase HK1	-
GP477_RS03130	696907..697377	-	471	WP_003403144.1	HAMP domain-containing protein	-
GP477_RS03135	697421..698182	-	762	WP_003403148.1	two-component system response regulator TcrA	-
GP477_RS03140	698239..698550	+	312	WP_003403164.1	hypothetical protein	-
GP477_RS03145	698622..699572	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
GP477_RS03150	699813..700397	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
GP477_RS03155	700399..701109	+	711	Protein_625	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T144771 WP_003403137.1 NZ_CP046728:c695950-695537 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T144771 NZ_CP062240:c3227814-3227500 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT144771 WP_003403139.1 NZ_CP046728:c696183-695947 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT144771 NZ_CP062240:c3227503-3227087 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89