Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 693222..693868 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag GP477_RS03100 Protein ID WP_003403122.1
Coordinates 693222..693614 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag GP477_RS03105 Protein ID WP_003403125.1
Coordinates 693611..693868 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS03085 688884..690410 + 1527 WP_003403112.1 virulence factor Mce family protein -
GP477_RS03090 690407..691615 + 1209 WP_003403114.1 virulence factor Mce family protein -
GP477_RS03095 691620..693170 + 1551 WP_003905349.1 MCE family protein -
GP477_RS03100 693222..693614 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
GP477_RS03105 693611..693868 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
GP477_RS03110 694051..695286 - 1236 WP_003403128.1 ATP-binding protein -
GP477_RS03115 695537..695950 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS03120 695947..696183 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GP477_RS03125 696287..696793 - 507 WP_003900973.1 two component system sensor kinase HK1 -
GP477_RS03130 696907..697377 - 471 WP_003403144.1 HAMP domain-containing protein -
GP477_RS03135 697421..698182 - 762 WP_003403148.1 two-component system response regulator TcrA -
GP477_RS03140 698239..698550 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T144770 WP_003403122.1 NZ_CP046728:c693614-693222 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T144770 NZ_CP062240:c3161903-3161607 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT144770 WP_003403125.1 NZ_CP046728:c693868-693611 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT144770 NZ_CP062240:c3161605-3161210 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References