Detailed information of TA system TA144763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 330976..331610
• Replicon: chromosome
• Accession: NZ_CP046728
• Organism: Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF84
• Locus tag: GP477_RS01465
• Protein ID: WP_003401406.1
• Coordinates: 330976..331404 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GP477_RS01470
• Protein ID: WP_011961607.1
• Coordinates: 331428..331610 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GP477_RS01440	326843..327976	-	1134	WP_003401394.1	hypothetical protein	-
GP477_RS01445	327973..328593	-	621	WP_003401397.1	TetR/AcrR family transcriptional regulator	-
GP477_RS01450	328690..329271	+	582	WP_003401399.1	VOC family protein	-
GP477_RS01455	329201..329926	-	726	WP_003899886.1	TetR/AcrR family transcriptional regulator	-
GP477_RS01460	330016..330936	+	921	WP_003401404.1	DUF2236 domain-containing protein	-
GP477_RS01465	330976..331404	-	429	WP_003401406.1	type II toxin-antitoxin system VapC family toxin	Toxin
GP477_RS01470	331428..331610	-	183	WP_011961607.1	hypothetical protein	Antitoxin
GP477_RS01475	331705..334512	-	2808	WP_025202582.1	PE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15887.27 Da
• Isoelectric Point: 7.2214

>T144763 WP_003401406.1 NZ_CP046728:c331404-330976 [Mycobacterium tuberculosis]
MFLIDVNVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLTTNRRIFEIPSPRADAFAFVEAVNAQPHHL
PTSPGPRHLVLLRKLCDEADASGDLIPDAVLGAIAVEHHCAVVSLDRDFARFASVRHIRPPI

Nucleotide

• Length: 429 bp

>T144763 NZ_CP062240:2071050-2071424 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6262.06 Da
• Isoelectric Point: 4.5878

>AT144763 WP_011961607.1 NZ_CP046728:c331610-331428 [Mycobacterium tuberculosis]
MGAVIEDALRRELAAARTGGARPTVPVFDAGTGPRPGIDLTSNTVLSEVLDEGLELNSRK

Nucleotide

• Length: 183 bp

>AT144763 NZ_CP062240:2070593-2070961 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BRM6