Detailed information of TA system TA144761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 38038..38591
• Replicon: plasmid pASB05p3
• Accession: NZ_CP046725
• Organism: Pantoea agglomerans strain ASB05

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: PAASB05_RS22305
• Protein ID: WP_158132510.1
• Coordinates: 38277..38591 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A7X2STP4
• Locus tag: PAASB05_RS22300
• Protein ID: WP_033781302.1
• Coordinates: 38038..38274 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAASB05_RS22270	34210..34500	-	291	WP_158132506.1	hypothetical protein	-
PAASB05_RS22275	34512..34739	-	228	Protein_28	DNA polymerase V subunit UmuC	-
PAASB05_RS22280	34742..35119	-	378	WP_158132507.1	peptidase	-
PAASB05_RS22285	35257..35937	+	681	WP_158132508.1	SOS response-associated peptidase family protein	-
PAASB05_RS22290	36393..36842	+	450	WP_158132509.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
PAASB05_RS22295	36920..37618	+	699	Protein_32	DUF4113 domain-containing protein	-
PAASB05_RS22300	38038..38274	+	237	WP_033781302.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
PAASB05_RS22305	38277..38591	+	315	WP_158132510.1	CcdB family protein	Toxin
PAASB05_RS22310	38732..38962	-	231	WP_158132535.1	hypothetical protein	-
PAASB05_RS22315	39066..39269	-	204	WP_031594620.1	hypothetical protein	-
PAASB05_RS22320	39266..39889	-	624	WP_031594621.1	AAA family ATPase	-
PAASB05_RS22325	40184..40978	-	795	WP_158132511.1	site-specific integrase	-
PAASB05_RS22330	40971..41519	-	549	WP_158132512.1	hypothetical protein	-
PAASB05_RS22335	41617..42066	-	450	WP_124890871.1	hypothetical protein	-
PAASB05_RS22340	42150..43085	-	936	WP_124890869.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64606	64606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11658.60 Da
• Isoelectric Point: 5.8036

>T144761 WP_158132510.1 NZ_CP046725:38277-38591 [Pantoea agglomerans]
MQFTVYSNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPLEKYPSSTRPERLVPLIRLTDDKEYAVMTHEMASIPTRALG
AVFCDSSIYRTEIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T144761 NZ_CP062240:c1639890-1639603 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8966.90 Da
• Isoelectric Point: 4.6234

>AT144761 WP_033781302.1 NZ_CP046725:38038-38274 [Pantoea agglomerans]
MAITQRATQSVTMTVERDLLSRAREAGINLSATLTTALDAELRQYEAKKWQEENSEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT144761 NZ_CP062240:c1640129-1639890 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X2STP4