Detailed information of TA system TA144754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3057835..3058452
• Replicon: chromosome
• Accession: NZ_CP046722
• Organism: Pantoea agglomerans strain ASB05

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: PAASB05_RS14335
• Protein ID: WP_158131946.1
• Coordinates: 3058237..3058452 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: E0LTU8
• Locus tag: PAASB05_RS14330
• Protein ID: WP_003850455.1
• Coordinates: 3057835..3058212 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAASB05_RS14300	3054142..3054396	+	255	WP_010671219.1	type B 50S ribosomal protein L31	-
PAASB05_RS14305	3054408..3054548	+	141	WP_010255896.1	type B 50S ribosomal protein L36	-
PAASB05_RS14310	3054594..3055472	-	879	WP_158131945.1	metal ABC transporter substrate-binding protein	-
PAASB05_RS14315	3055488..3056327	-	840	WP_010671217.1	metal ABC transporter permease	-
PAASB05_RS14320	3056324..3056992	-	669	WP_033766689.1	ABC transporter ATP-binding protein	-
PAASB05_RS14325	3057334..3057687	+	354	WP_010255885.1	hypothetical protein	-
PAASB05_RS14330	3057835..3058212	+	378	WP_003850455.1	Hha toxicity modulator TomB	Antitoxin
PAASB05_RS14335	3058237..3058452	+	216	WP_158131946.1	hemolysin expression modulator Hha	Toxin
PAASB05_RS14345	3058859..3059170	+	312	WP_033756781.1	MGMT family protein	-
PAASB05_RS14350	3059210..3059767	-	558	WP_010255881.1	YbaY family lipoprotein	-
PAASB05_RS14355	3059958..3060821	+	864	WP_010255880.1	acyl-CoA thioesterase II	-
PAASB05_RS14360	3060874..3062160	-	1287	WP_010255877.1	ammonium transporter AmtB	-
PAASB05_RS14365	3062195..3062533	-	339	WP_003850469.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (12-68)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8524.93 Da
• Isoelectric Point: 9.4825

>T144754 WP_158131946.1 NZ_CP046722:3058237-3058452 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEEELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Nucleotide

• Length: 216 bp

>T144754 NZ_CP062240:c475764-475546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14636.25 Da
• Isoelectric Point: 4.3976

>AT144754 WP_003850455.1 NZ_CP046722:3057835-3058212 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Nucleotide

• Length: 378 bp

>AT144754 NZ_CP062240:c476164-475790 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1I5AG55