Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3418356..3418974 Replicon chromosome
Accession NZ_CP046716
Organism Escherichia coli strain T16R

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GPL39_RS16435 Protein ID WP_001291435.1
Coordinates 3418756..3418974 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GPL39_RS16430 Protein ID WP_000344800.1
Coordinates 3418356..3418730 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPL39_RS16420 3413445..3414638 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GPL39_RS16425 3414661..3417810 + 3150 WP_113402280.1 efflux RND transporter permease AcrB -
GPL39_RS16430 3418356..3418730 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GPL39_RS16435 3418756..3418974 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GPL39_RS16440 3419146..3419697 + 552 WP_000102564.1 maltose O-acetyltransferase -
GPL39_RS16445 3419813..3420283 + 471 WP_000136192.1 YlaC family protein -
GPL39_RS16450 3420447..3421997 + 1551 WP_063501968.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GPL39_RS16455 3422039..3422392 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GPL39_RS16465 3422771..3423082 + 312 WP_000409911.1 MGMT family protein -
GPL39_RS16470 3423113..3423685 - 573 WP_113402281.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T144738 WP_001291435.1 NZ_CP046716:3418756-3418974 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T144738 NZ_CP062239:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT144738 WP_000344800.1 NZ_CP046716:3418356-3418730 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT144738 NZ_CP062239:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References