Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1260612..1261237 Replicon chromosome
Accession NZ_CP046716
Organism Escherichia coli strain T16R

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag GPL39_RS06035 Protein ID WP_000911331.1
Coordinates 1260839..1261237 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GPL39_RS06030 Protein ID WP_000450524.1
Coordinates 1260612..1260839 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPL39_RS06005 1256415..1256885 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
GPL39_RS06010 1256885..1257457 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GPL39_RS06015 1257603..1258481 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GPL39_RS06020 1258498..1259532 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GPL39_RS06025 1259745..1260458 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GPL39_RS06030 1260612..1260839 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GPL39_RS06035 1260839..1261237 + 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
GPL39_RS06040 1261384..1262247 + 864 WP_001267507.1 neutral zinc metallopeptidase -
GPL39_RS06045 1262262..1264277 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
GPL39_RS06050 1264351..1265049 + 699 WP_000679812.1 esterase -
GPL39_RS06055 1265159..1265359 - 201 WP_089631931.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T144726 WP_000911331.1 NZ_CP046716:1260839-1261237 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T144726 NZ_CP062239:c259380-259039 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT144726 WP_000450524.1 NZ_CP046716:1260612-1260839 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT144726 NZ_CP062239:c259718-259401 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References