Detailed information of TA system TA144723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 771741..772339
• Replicon: chromosome
• Accession: NZ_CP046716
• Organism: Escherichia coli strain T16R

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: GPL39_RS03740
• Protein ID: WP_053291281.1
• Coordinates: 771962..772339 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GPL39_RS03735
• Protein ID: WP_001603498.1
• Coordinates: 771741..771962 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPL39_RS03720	767341..768021	+	681	WP_053270475.1	fimbrial assembly chaperone	-
GPL39_RS03725	768039..770525	+	2487	WP_096218945.1	fimbria/pilus outer membrane usher protein	-
GPL39_RS03730	770537..771556	+	1020	WP_095784823.1	type 1 fimbrial protein	-
GPL39_RS03735	771741..771962	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPL39_RS03740	771962..772339	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GPL39_RS03745	772449..772664	-	216	Protein_731	transposase	-
GPL39_RS03750	772850..774112	-	1263	WP_113402210.1	integrase arm-type DNA-binding domain-containing protein	-
GPL39_RS03760	774491..775198	-	708	WP_000234517.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	756040..774112	18072
	-	flank	IS/Tn	-	-	772383..772664	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T144723 WP_053291281.1 NZ_CP046716:771962-772339 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T144723 NZ_CP062232:131212-132471 [Rhizobium sp. TH2]
GTGACTCGATACTTGGACGTCTATTTCAGCAACATAAAGGCCGGCACACTCGGCCAGGACGCCGACGGGACGCTTACCTA
CTCATACGACGTCGAGTATCTTCGAGATGGAGGAGTCGCGGCGATTTCCTTCTCAATGCCGTTGAGGGAAGAGCCCTACC
TCGACCAGATCGTTCGCCCATTCTTCTCGGGCTTGCTTCCCGATGAGGGCGCCCGACAGCGTCTTGCGGGCGCACTTGGC
CTCTCGTCCGGCAATGCATTCGGTTTACTCGAAGTGATCGGCGGCGAGTGTGCAGGTGCATTGTCTCTCCATCGCGCTGG
CGAGGCGCCTGCATTGAATGACGATGAGGGGGTCGAGGAGCTTAGCGGCGAACGACTGAGTGATGTGATCGGCAAATTGC
GCAGCCGTCCCCTCCTGGGTGGCGAGGAAGGGGTTCGCCTCTCGCTTGCGGGCGCGCAGGACAAGCTCGCGGTCATCGCG
CAAGACGACACCATTGGCTTGGCCAAGGGTGGCCGTCCGACAACCCACATTCTCAAACCGGTGATCCAAGGCCTGGAAGG
AACTGTCGAGAATGAGCTTTTTTGTTTGCGTCTTGCTGATCGCCTGAAACTACCGGTTCCTACTGCAGAGATGCGGTGGA
GCAACGAGACAGCCTTCTTGCTCATTGAACGTTATGACCGTGCCCGGGCGAGCCACGGACGTATTGACCGGTTGCACCAG
GAGGATTTTTGCCAGGCGCTGAGCGTGCCACCAGAACTCAAATACGAGGAGGAGGGCGGTCCTAGCACGGAACGCTCAGT
TGATCTCATCAACCGAGCCTGCGGTCGCCCGGCCGCAGACCGCCTACGCTTCATCAGGATGCTGATTTTTCACTACCTTG
TTGGCAATGCGGATGCCCACGGGAAGAACTATGCCTTACTCTATGACGGTAAGTCTCCAGACCTTGCGCCGCTCTATGAT
GTGGTTTGCACAGCCGCCTATCCCAGACTGGCCAAGAAGCTCGCAATGAAGATCGGTGGTCGGGCGGTTCCAGACACGAC
CCAACTGAAACACTGGCTGACCCTCGTCCCCGACACAAAGGCGGCACAACGTCTTCTCGTCAGCGATATCGCAAATCTCG
CTGGCAACATTGAAAGCGAAGCCGACGCGCTGTTGCTTGAGTTCTCGGATGCGGGCGTCAAGCACCCGGTCCTCAAAGCA
GTGCGCGGAATTGTTGGAACCCGCGCCGCACATCTGCAGAGGATTATCGAGAGGGTGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT144723 WP_001603498.1 NZ_CP046716:771741-771962 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT144723 NZ_CP062232:130988-131209 [Rhizobium sp. TH2]
ATGGACACTGTTAGCGACACCAATACGCTTGGAATGCTTATTCGGGCTGAGAGAAAATCTCAGAGCCTCACCCAAGAGCA
GCTTGCCGGCATTGCTGGAGTTGGTGTTCGATTTGTGCGTGAGCTCGAGGCCGGCAAGGAGAGCTGTCAACTTGGTCGAG
CGTTGCAGGTGGCCCAAACACTCGGCCTTGTCCTGTCTGTAAGCACCAGACGGGAGAGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0