Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 362903..363536 Replicon chromosome
Accession NZ_CP046674
Organism Ralstonia nicotianae strain RS

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag GO999_RS01595 Protein ID WP_016725172.1
Coordinates 363123..363536 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag GO999_RS01590 Protein ID WP_011002949.1
Coordinates 362903..363136 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO999_RS01575 (GO999_01575) 358491..359786 - 1296 WP_011002952.1 MFS transporter -
GO999_RS01580 (GO999_01580) 359794..361026 - 1233 WP_231679073.1 AGE family epimerase/isomerase -
GO999_RS01585 (GO999_01585) 361239..362846 + 1608 WP_211906500.1 glucan biosynthesis protein -
GO999_RS01590 (GO999_01590) 362903..363136 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GO999_RS01595 (GO999_01595) 363123..363536 + 414 WP_016725172.1 PIN domain-containing protein Toxin
GO999_RS01600 (GO999_01600) 363511..364137 - 627 WP_016725173.1 DUF1415 domain-containing protein -
GO999_RS01605 (GO999_01605) 364328..364699 + 372 WP_211906501.1 hypothetical protein -
GO999_RS01610 (GO999_01610) 364760..366256 - 1497 WP_011002945.1 glycerol kinase GlpK -
GO999_RS01615 (GO999_01615) 366418..368154 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T144713 WP_016725172.1 NZ_CP046674:363123-363536 [Ralstonia nicotianae]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T144713 NZ_CP062229:6604542-6604979 [Mesorhizobium onobrychidis]
GTGACCTTCCTGCTCGACGTCAATGTACTGATCGCGCTGATCGATCCTGGCCATGTCGGCCATGACGACGCGCATGGCTG
GTTCGAAGCGACCGGTCACGCCGCATGGGCCACTTGTCCCATCACCGAAAACGGCGTCATCCGCATCATCGGCAATCCGA
AATATCCCAATTCTCCCGGTTCGCCTGCTGTCGTCGCGCAGATCGTTACTAAGTTACGCGCCCTGCCCGGCCACAGCTTC
TGGCCCGACGATGTGAGCCTCGTCGGCTCAGGCGACATTGATGCGGCAAAAGTCCTGACTTCAGCACAGGTTACCGACAT
CTATCTTCTTGCGCTCGCTAAGGCGCATAGTGGCCATCTGGCGAGCTTCGACCGCAAGCTGTCGACGGCGGCCGTGAGAG
GCGGGAAATCCGCACTGCATCTGATCCCCGCAAGATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT144713 WP_011002949.1 NZ_CP046674:362903-363136 [Ralstonia nicotianae]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT144713 NZ_CP062229:6604318-6604545 [Mesorhizobium onobrychidis]
ATGAGAACGACACTAGCGATAGATGACGACGTCCTGCTCGCGGCCAAGGCCATGGCCCGGCAGCAGGATCGCAGCGTCGG
TGAGATCATCACCGATCTCGCCCGGCGCTCACTGCGCCGCCCGCAGGCCGGCGGCGAGCGCAACGGGATTCCTCTGCTGT
CGCCTCGGTCGGATACACCTCCTGTCACGCTTGAGACGGTCAATGCCTTGCGTGACAAGCTGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References