Detailed information of TA system TA144710

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4190686..4191340
• Replicon: chromosome
• Accession: NZ_CP046673
• Organism: Citrobacter sp. 172116965

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: GPJ68_RS20330
• Protein ID: WP_104651977.1
• Coordinates: 4190686..4191093 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: GPJ68_RS20335
• Protein ID: WP_016154349.1
• Coordinates: 4191074..4191340 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ68_RS20310	4186578..4188311	-	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-
GPJ68_RS20315	4188317..4189030	-	714	WP_047414418.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GPJ68_RS20320	4189054..4189950	-	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
GPJ68_RS20325	4190064..4190585	+	522	WP_016154347.1	flavodoxin FldB	-
GPJ68_RS20330	4190686..4191093	-	408	WP_104651977.1	protein YgfX	Toxin
GPJ68_RS20335	4191074..4191340	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
GPJ68_RS20340	4191598..4192578	+	981	WP_094168631.1	tRNA-modifying protein YgfZ	-
GPJ68_RS20345	4192658..4193317	-	660	WP_016154351.1	hemolysin III family protein	-
GPJ68_RS20350	4193481..4193792	-	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
GPJ68_RS20355	4193845..4194573	+	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
GPJ68_RS20360	4194695..4196128	+	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15900.73 Da
• Isoelectric Point: 11.7416

>T144710 WP_104651977.1 NZ_CP046673:c4191093-4190686 [Citrobacter sp. 172116965]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSHAGKRQHLWLAADSMDDAEWRDLRRLILQQARQG

Nucleotide

• Length: 408 bp

>T144710 NZ_CP062229:c2533954-2533661 [Mesorhizobium onobrychidis]
ATGAAGCGAACCGTCAGGCTCTCACCGAAAGCGAAAGCAGATCTCGACGACATTTGGGATCATTCGTTGAAAGATTGGGG
CTTAGAGCGGGCGGAAGCCTATATTCTCACCGTTCATTCCATAATCAATCTGATAGACCAATTTCCGGCAATGGCGAAAA
ACGCAGAGAACATTCGCCCCGGTCTCTTGAAATATCCCATTGGCTCTCTCGTTCTGTTTCTGCGAATCGGCGATCAGTAC
ATCGATATTGTACGCATTCTGCATCAGCGCCTGGACTATCCCAGGCATCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT144710 WP_016154349.1 NZ_CP046673:c4191340-4191074 [Citrobacter sp. 172116965]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT144710 NZ_CP062229:c2534187-2533951 [Mesorhizobium onobrychidis]
ATGTCCAAGAATACCTCCATCACACTAGGCGACCAGTACGATGCCTTTATCAAGCGGCAGATAGCAAATGGCCGCTTCGG
TTCGGCAAGCGAGACCGTTCGCGCTGGCCTGCGTCTTTTAGAAGAACAGGAGCTGAAGCTTGAGCAACTCCGCGCCGCCA
TTGATGAGGGTGACATGTCAGGTGACCCCGAACCGTTCGACTTCGAGGCTTTTCTCAAATCCAAAAAGCGCGGATGA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6