Detailed information of TA system TA144709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2557190..2557826
• Replicon: chromosome
• Accession: NZ_CP046673
• Organism: Citrobacter sp. 172116965

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: GPJ68_RS12365
• Protein ID: WP_049259794.1
• Coordinates: 2557638..2557826 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GPJ68_RS12360
• Protein ID: WP_131382557.1
• Coordinates: 2557190..2557606 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ68_RS12340	2552235..2553179	-	945	WP_016153152.1	ABC transporter permease	-
GPJ68_RS12345	2553180..2554196	-	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
GPJ68_RS12350	2554213..2555358	-	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
GPJ68_RS12355	2555688..2557097	-	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
GPJ68_RS12360	2557190..2557606	-	417	WP_131382557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GPJ68_RS12365	2557638..2557826	-	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
GPJ68_RS12370	2558090..2559493	+	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
GPJ68_RS12375	2559493..2560503	+	1011	WP_180837399.1	cytochrome d ubiquinol oxidase subunit II	-
GPJ68_RS12380	2560503..2560628	+	126	WP_104651600.1	DUF2474 domain-containing protein	-
GPJ68_RS12385	2560818..2561048	+	231	WP_019076831.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T144709 WP_049259794.1 NZ_CP046673:c2557826-2557638 [Citrobacter sp. 172116965]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T144709 NZ_CP062228:4895344-4895721 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.33 Da
• Isoelectric Point: 4.7119

>AT144709 WP_131382557.1 NZ_CP046673:c2557606-2557190 [Citrobacter sp. 172116965]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT144709 NZ_CP062228:4894886-4895254 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9