Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1277158..1277778 Replicon chromosome
Accession NZ_CP046673
Organism Citrobacter sp. 172116965

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GPJ68_RS06070 Protein ID WP_002892050.1
Coordinates 1277158..1277376 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag GPJ68_RS06075 Protein ID WP_003021733.1
Coordinates 1277404..1277778 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ68_RS06035 1272517..1273146 + 630 WP_016155872.1 membrane protein -
GPJ68_RS06040 1273211..1273564 + 354 WP_003831033.1 DUF1428 family protein -
GPJ68_RS06045 1273616..1275169 - 1554 WP_180837176.1 EAL domain-containing protein -
GPJ68_RS06050 1275358..1275618 + 261 WP_104651311.1 type B 50S ribosomal protein L31 -
GPJ68_RS06055 1275620..1275760 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
GPJ68_RS06060 1275839..1276309 - 471 WP_016152071.1 YlaC family protein -
GPJ68_RS06065 1276426..1276977 - 552 WP_103283555.1 maltose O-acetyltransferase -
GPJ68_RS06070 1277158..1277376 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GPJ68_RS06075 1277404..1277778 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
GPJ68_RS06080 1278266..1281415 - 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
GPJ68_RS06085 1281438..1282631 - 1194 WP_047416860.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T144700 WP_002892050.1 NZ_CP046673:c1277376-1277158 [Citrobacter sp. 172116965]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T144700 NZ_CP062228:c2227872-2227618 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT144700 WP_003021733.1 NZ_CP046673:c1277778-1277404 [Citrobacter sp. 172116965]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT144700 NZ_CP062228:c2228120-2227869 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References