Detailed information of TA system TA144698

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4932861..4933633
• Replicon: chromosome
• Accession: NZ_CP046672
• Organism: Raoultella ornithinolytica strain 172117885

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8B4TZ74
• Locus tag: GPJ66_RS23145
• Protein ID: WP_044347594.1
• Coordinates: 4932861..4933250 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GPJ66_RS23150
• Protein ID: WP_177339494.1
• Coordinates: 4933307..4933633 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ66_RS23125	4928352..4929173	+	822	WP_162661732.1	hypothetical protein	-
GPJ66_RS23130	4929438..4930700	-	1263	Protein_4534	integrase arm-type DNA-binding domain-containing protein	-
GPJ66_RS23135	4930982..4931779	-	798	WP_044347598.1	helix-turn-helix transcriptional regulator	-
GPJ66_RS23140	4931929..4932774	-	846	WP_044347596.1	DUF4942 domain-containing protein	-
GPJ66_RS23145	4932861..4933250	-	390	WP_044347594.1	TA system toxin CbtA family protein	Toxin
GPJ66_RS23150	4933307..4933633	-	327	WP_177339494.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GPJ66_RS23155	4933691..4933912	-	222	WP_044347590.1	DUF987 domain-containing protein	-
GPJ66_RS23160	4933926..4934405	-	480	WP_044347588.1	DNA repair protein RadC	-
GPJ66_RS23165	4934417..4934860	-	444	WP_044347586.1	antirestriction protein	-
GPJ66_RS23170	4934940..4935188	-	249	WP_044347584.1	DUF905 domain-containing protein	-
GPJ66_RS23175	4935194..4936018	-	825	WP_044347582.1	DUF945 domain-containing protein	-
GPJ66_RS23180	4936398..4937339	-	942	WP_049109197.1	hypothetical protein	-
GPJ66_RS23185	4937915..4938535	+	621	WP_049109196.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4922211..4954915	32704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14386.44 Da
• Isoelectric Point: 9.8627

>T144698 WP_044347594.1 NZ_CP046672:c4933250-4932861 [Raoultella ornithinolytica]
MQTKSSPLMRAASSRPSPVYIWQTLLTSLLANHYGLTLSDTPFSDEQVIQQHIDAGISLADALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKAAGLMNRDTYKEVTAITRGQHPQVNTSGKR

Nucleotide

• Length: 390 bp

>T144698 NZ_CP062228:c1949896-1949794 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12126.78 Da
• Isoelectric Point: 6.3156

>AT144698 WP_177339494.1 NZ_CP046672:c4933633-4933307 [Raoultella ornithinolytica]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLDDRANFDGQFCDADLRHLDQAFPVLLKQLELMLTSGELNPRHQHGVTLYA
KGLICEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT144698 NZ_CP062228:1949756-1950024 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A8B4TZ74