Detailed information of TA system TA144697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4839478..4840054
• Replicon: chromosome
• Accession: NZ_CP046672
• Organism: Raoultella ornithinolytica strain 172117885

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: GPJ66_RS22725
• Protein ID: WP_032716505.1
• Coordinates: 4839767..4840054 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: GPJ66_RS22720
• Protein ID: WP_113991318.1
• Coordinates: 4839478..4839780 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ66_RS22705	4835421..4835756	+	336	WP_069476269.1	endoribonuclease SymE	-
GPJ66_RS22710	4836084..4838060	+	1977	WP_180862668.1	AAA family ATPase	-
GPJ66_RS22715	4838060..4839382	+	1323	WP_162661703.1	restriction endonuclease	-
GPJ66_RS22720	4839478..4839780	-	303	WP_113991318.1	BrnA antitoxin family protein	Antitoxin
GPJ66_RS22725	4839767..4840054	-	288	WP_032716505.1	BrnT family toxin	Toxin
GPJ66_RS22730	4840171..4840590	-	420	WP_004857515.1	FosA family fosfomycin resistance glutathione transferase	-
GPJ66_RS22735	4840584..4841492	-	909	WP_180862396.1	LysR family transcriptional regulator	-
GPJ66_RS22740	4841580..4842362	+	783	WP_044348932.1	NAD(P)H-dependent oxidoreductase	-
GPJ66_RS22745	4842510..4843088	+	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
GPJ66_RS22750	4843232..4844044	+	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
GPJ66_RS22755	4844041..4844559	+	519	WP_015585229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T144697 WP_032716505.1 NZ_CP046672:c4840054-4839767 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T144697 NZ_CP062228:c1664002-1662680 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11539.05 Da
• Isoelectric Point: 7.0165

>AT144697 WP_113991318.1 NZ_CP046672:c4839780-4839478 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLSEK

Nucleotide

• Length: 303 bp

>AT144697 NZ_CP062228:c1664268-1664002 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A808YBH6