Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4251183..4251802 Replicon chromosome
Accession NZ_CP046672
Organism Raoultella ornithinolytica strain 172117885

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag GPJ66_RS20005 Protein ID WP_004858783.1
Coordinates 4251584..4251802 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag GPJ66_RS20000 Protein ID WP_004858785.1
Coordinates 4251183..4251557 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ66_RS19990 4246293..4247486 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GPJ66_RS19995 4247509..4250655 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
GPJ66_RS20000 4251183..4251557 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
GPJ66_RS20005 4251584..4251802 + 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
GPJ66_RS20010 4251954..4252520 + 567 WP_004858781.1 maltose O-acetyltransferase -
GPJ66_RS20015 4252620..4253090 + 471 WP_004858780.1 YlaC family protein -
GPJ66_RS20020 4253065..4254516 - 1452 WP_104896844.1 PLP-dependent aminotransferase family protein -
GPJ66_RS20025 4254621..4255331 + 711 WP_015585015.1 GNAT family N-acetyltransferase -
GPJ66_RS20030 4255328..4255468 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
GPJ66_RS20035 4255471..4255731 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T144696 WP_004858783.1 NZ_CP046672:4251584-4251802 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T144696 NZ_CP062228:c1619554-1619276 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT144696 WP_004858785.1 NZ_CP046672:4251183-4251557 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT144696 NZ_CP062228:c1619276-1618992 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References