Detailed information of TA system TA144683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 67575..68122
• Replicon: chromosome
• Accession: NZ_CP046672
• Organism: Raoultella ornithinolytica strain 172117885

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GPJ66_RS00315
• Protein ID: WP_123629131.1
• Coordinates: 67575..67883 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A808XYT1
• Locus tag: GPJ66_RS00320
• Protein ID: WP_004869059.1
• Coordinates: 67886..68122 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ66_RS00285	62754..63056	-	303	WP_015585486.1	PTS lactose/cellobiose transporter subunit IIA	-
GPJ66_RS00290	63191..64516	-	1326	WP_004869064.1	PTS cellobiose transporter subunit IIC	-
GPJ66_RS00295	64528..64842	-	315	WP_004869063.1	PTS sugar transporter subunit IIB	-
GPJ66_RS00300	65137..66078	+	942	WP_015585487.1	LacI family transcriptional regulator	-
GPJ66_RS00305	66173..66448	-	276	WP_160873538.1	hypothetical protein	-
GPJ66_RS00310	66557..67459	-	903	WP_015585496.1	EamA family transporter	-
GPJ66_RS00315	67575..67883	-	309	WP_123629131.1	CcdB family protein	Toxin
GPJ66_RS00320	67886..68122	-	237	WP_004869059.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GPJ66_RS00325	68228..69661	-	1434	WP_069476354.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
GPJ66_RS00330	69686..70588	-	903	WP_044350979.1	N-acetylmuramic acid 6-phosphate etherase	-
GPJ66_RS00335	70753..71916	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11792.64 Da
• Isoelectric Point: 6.7156

>T144683 WP_123629131.1 NZ_CP046672:c67883-67575 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRIVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Nucleotide

• Length: 309 bp

>T144683 NZ_CP062219:c4922818-4922537 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8898.08 Da
• Isoelectric Point: 5.6579

>AT144683 WP_004869059.1 NZ_CP046672:c68122-67886 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT144683 NZ_CP062219:c4923042-4922815 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808XYT1