Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4906521..4907100 Replicon chromosome
Accession NZ_CP046671
Organism Raoultella ornithinolytica strain 193104922

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag GPJ65_RS23335 Protein ID WP_015585223.1
Coordinates 4906813..4907100 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag GPJ65_RS23330 Protein ID WP_015585222.1
Coordinates 4906521..4906826 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ65_RS23305 4901729..4902793 - 1065 WP_004857527.1 DUF2955 domain-containing protein -
GPJ65_RS23310 4902783..4903850 - 1068 WP_004857525.1 HlyD family secretion protein -
GPJ65_RS23315 4903857..4904321 - 465 WP_004857523.1 MarR family transcriptional regulator -
GPJ65_RS23320 4904612..4904776 - 165 WP_004857521.1 DUF1127 domain-containing protein -
GPJ65_RS23325 4904954..4906366 + 1413 WP_015585221.1 PLP-dependent aminotransferase family protein -
GPJ65_RS23330 4906521..4906826 - 306 WP_015585222.1 BrnA antitoxin family protein Antitoxin
GPJ65_RS23335 4906813..4907100 - 288 WP_015585223.1 BrnT family toxin Toxin
GPJ65_RS23340 4907217..4907636 - 420 WP_015585224.1 FosA family fosfomycin resistance glutathione transferase -
GPJ65_RS23345 4907630..4908538 - 909 WP_015585225.1 LysR family transcriptional regulator -
GPJ65_RS23350 4908626..4909408 + 783 WP_044348932.1 NAD(P)H-dependent oxidoreductase -
GPJ65_RS23355 4909556..4910134 + 579 WP_004857495.1 TetR/AcrR family transcriptional regulator -
GPJ65_RS23360 4910278..4911090 + 813 WP_004857492.1 winged helix-turn-helix domain-containing protein -
GPJ65_RS23365 4911087..4911605 + 519 WP_015585229.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(40-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11067.49 Da        Isoelectric Point: 7.0747

>T144682 WP_015585223.1 NZ_CP046671:c4907100-4906813 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRQDRYENGEHRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADGKERSRYEHS

Download         Length: 288 bp

>T144682 NZ_CP062219:c3731026-3730748 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11526.01 Da        Isoelectric Point: 9.7545

>AT144682 WP_015585222.1 NZ_CP046671:c4906826-4906521 [Raoultella ornithinolytica]
MSTVKYKRDNPAGLSAQREDELRALANKADGEIDYSDIPASGDEQWPDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Download         Length: 306 bp

>AT144682 NZ_CP062219:c3730736-3730431 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References