Detailed information of TA system TA144680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2981493..2982244
• Replicon: chromosome
• Accession: NZ_CP046671
• Organism: Raoultella ornithinolytica strain 193104922

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GPJ65_RS14035
• Protein ID: WP_126509690.1
• Coordinates: 2981493..2981975 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: GPJ65_RS14040
• Protein ID: WP_015584326.1
• Coordinates: 2981966..2982244 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ65_RS14010	2976872..2977747	+	876	WP_032717451.1	NAD(P)-dependent oxidoreductase	-
GPJ65_RS14015	2978407..2978565	+	159	WP_004861965.1	hypothetical protein	-
GPJ65_RS14020	2978759..2980162	+	1404	WP_057061543.1	ISNCY-like element ISKpn21 family transposase	-
GPJ65_RS14025	2980191..2980823	-	633	WP_001567369.1	hypothetical protein	-
GPJ65_RS14030	2980958..2981308	-	351	WP_044351090.1	hypothetical protein	-
GPJ65_RS14035	2981493..2981975	-	483	WP_126509690.1	GNAT family N-acetyltransferase	Toxin
GPJ65_RS14040	2981966..2982244	-	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
GPJ65_RS14045	2982875..2984800	+	1926	WP_180849961.1	C1 family peptidase	-
GPJ65_RS14050	2984958..2985455	-	498	WP_048023692.1	hypothetical protein	-
GPJ65_RS14055	2985671..2987038	-	1368	Protein_2755	KUP/HAK/KT family potassium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaPLA1a	iroN	2959081..3359280	400199
	-	flank	IS/Tn	-	-	2978759..2980162	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17616.47 Da
• Isoelectric Point: 9.7381

>T144680 WP_126509690.1 NZ_CP046671:c2981975-2981493 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVTGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T144680 NZ_CP046671:c2981975-2981493 [Raoultella ornithinolytica]
ATGGGAATGAGACCACCGGAACCACTCAGCGCTGGACACAACATAGCAGAATTTTGTTGTCAGGATCAGGTTCTTAACGA
GTGGTTGAAGAAGAAGGCGCTGAAGAACCACAGTACAGGCATTTCAAGGGTCTTTGTGGTCTGTGCCGAAAACACAAAAA
GGGTCATCGCGTATTACAGCTTGTCATCCGGGAGCGTGCACAGAAACACAGTAACGGGGGCATACCGTCGTAATGCACCT
GAAGCTGTACCGGTCATTGTTCTGGGAAGGCTTGCGGTCGATGCAGCCTGGGCAGGGAAGGGACTCGGAGCGGCACTGCT
TAAGGATGCAATATACCGTACAGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTCCATGCCTTGAATGATG
AAGTCAGGGAGTTTTACACCAGGTTTGGATTCGAGCCCTCGATTATCAATACACTGACATTATTGTTCCCGATAAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT144680 WP_015584326.1 NZ_CP046671:c2982244-2981966 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT144680 NZ_CP046671:c2982244-2981966 [Raoultella ornithinolytica]
ATGAAACCAGACGTTCGTGAAGCTCCGATAAACATTCGGGCGAAAACATCTCAGCGCGACCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTTGAAGTGGCCTGTCGCGAAGCTCAGGACATTCTGTTGGATCAGCGCT
TGTTCCTGCTGGACGATAAGCAGTTTGCTAGTTTCATGGAAGAGCTTGACGCACCTATGACAGCGGAGCGTCAGGCACGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8