Detailed information of TA system TA144679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2968000..2969603
• Replicon: chromosome
• Accession: NZ_CP046671
• Organism: Raoultella ornithinolytica strain 193104922

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPJ65_RS13975
• Protein ID: WP_064367563.1
• Coordinates: 2968272..2969603 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: GPJ65_RS13970
• Protein ID: WP_032717448.1
• Coordinates: 2968000..2968272 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ65_RS13945	2963619..2964860	+	1242	WP_004861912.1	sensor domain-containing diguanylate cyclase	-
GPJ65_RS13950	2964880..2965512	+	633	WP_048023555.1	class I SAM-dependent methyltransferase	-
GPJ65_RS13955	2965726..2966679	+	954	WP_004861921.1	hypothetical protein	-
GPJ65_RS13960	2966812..2967237	+	426	WP_032724724.1	hypothetical protein	-
GPJ65_RS13965	2967243..2967797	+	555	WP_004861926.1	RNA polymerase sigma factor	-
GPJ65_RS13970	2968000..2968272	+	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GPJ65_RS13975	2968272..2969603	+	1332	WP_064367563.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPJ65_RS13980	2969536..2970951	-	1416	WP_099144431.1	glycoside hydrolase family 10 protein	-
GPJ65_RS13985	2971657..2972715	+	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
GPJ65_RS13990	2972815..2973729	+	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaPLA1a	iroN	2959081..3359280	400199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49313.63 Da
• Isoelectric Point: 6.7010

>T144679 WP_064367563.1 NZ_CP046671:2968272-2969603 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGDQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T144679 NZ_CP062219:117120-117707 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT144679 WP_032717448.1 NZ_CP046671:2968000-2968272 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT144679 NZ_CP062219:117774-118628 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGTCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5