Detailed information of TA system TA144660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3257267..3257903
• Replicon: chromosome
• Accession: NZ_CP046653
• Organism: Bacillus sp. ms-22

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: GPA07_RS16985
• Protein ID: WP_003214169.1
• Coordinates: 3257267..3257617 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K2P1A0
• Locus tag: GPA07_RS16990
• Protein ID: WP_008359553.1
• Coordinates: 3257622..3257903 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPA07_RS16945	3252297..3252896	-	600	WP_008359571.1	SpoIIE family protein phosphatase	-
GPA07_RS16950	3252896..3253684	-	789	WP_008359569.1	RNA polymerase sigma factor SigB	-
GPA07_RS16955	3253650..3254138	-	489	WP_008359566.1	anti-sigma B factor RsbW	-
GPA07_RS16960	3254135..3254464	-	330	WP_008359565.1	anti-sigma factor antagonist	-
GPA07_RS16965	3254524..3255531	-	1008	WP_008359562.1	PP2C family protein-serine/threonine phosphatase	-
GPA07_RS16970	3255542..3255943	-	402	WP_008359561.1	anti-sigma regulatory factor	-
GPA07_RS16975	3255946..3256314	-	369	WP_008359559.1	RsbT antagonist protein RsbS	-
GPA07_RS16980	3256319..3257152	-	834	WP_174549250.1	RsbT co-antagonist protein RsbRA	-
GPA07_RS16985	3257267..3257617	-	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GPA07_RS16990	3257622..3257903	-	282	WP_008359553.1	hypothetical protein	Antitoxin
GPA07_RS16995	3258200..3259378	-	1179	WP_157293024.1	alanine racemase	-
GPA07_RS17000	3259519..3260535	-	1017	WP_145939394.1	outer membrane lipoprotein-sorting protein	-
GPA07_RS17005	3260696..3261061	-	366	WP_008359547.1	holo-ACP synthase	-
GPA07_RS17010	3261156..3261761	+	606	WP_008359545.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T144660 WP_003214169.1 NZ_CP046653:c3257617-3257267 [Bacillus sp. ms-22]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T144660 NZ_CP062211:c3377896-3377498 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10836.19 Da
• Isoelectric Point: 4.9446

>AT144660 WP_008359553.1 NZ_CP046653:c3257903-3257622 [Bacillus sp. ms-22]
MSESSATNEMKIQLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT144660 NZ_CP062211:c3378123-3377896 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: K2P1A0