Detailed information of TA system TA144659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel2-ATphRel2/DUF4065(antitoxin)
• Location: 2613590..2615049
• Replicon: chromosome
• Accession: NZ_CP046653
• Organism: Bacillus sp. ms-22

Toxin (Protein)

• Gene name: phRel2
• Uniprot ID: -
• Locus tag: GPA07_RS13600
• Protein ID: WP_157292747.1
• Coordinates: 2614342..2615049 (-)
• Length: 236 a.a.

Antitoxin (Protein)

• Gene name: ATphRel2
• Uniprot ID: -
• Locus tag: GPA07_RS13595
• Protein ID: WP_157292745.1
• Coordinates: 2613590..2614339 (-)
• Length: 250 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPA07_RS13580	2610302..2611363	-	1062	WP_157292739.1	toll/interleukin-1 receptor domain-containing protein	-
GPA07_RS13585	2611511..2612344	+	834	WP_157292741.1	DUF2971 domain-containing protein	-
GPA07_RS13590	2612496..2613335	-	840	WP_157292743.1	SMI1/KNR4 family protein	-
GPA07_RS13595	2613590..2614339	-	750	WP_157292745.1	DUF4065 domain-containing protein	Antitoxin
GPA07_RS13600	2614342..2615049	-	708	WP_157292747.1	hypothetical protein	Toxin
GPA07_RS13605	2615457..2615708	+	252	WP_157292749.1	hypothetical protein	-
GPA07_RS13610	2615754..2616566	-	813	WP_157292751.1	N-acetylmuramoyl-L-alanine amidase	-
GPA07_RS13615	2616623..2616886	-	264	WP_157292753.1	phage holin	-
GPA07_RS13620	2616899..2617111	-	213	WP_034620157.1	hypothetical protein	-
GPA07_RS13625	2617173..2617316	-	144	WP_198303822.1	XkdX family protein	-
GPA07_RS13630	2617313..2617687	-	375	WP_157292755.1	hypothetical protein	-
GPA07_RS13635	2617693..2618823	-	1131	WP_157292756.1	hypothetical protein	-
GPA07_RS13640	2618841..2619224	-	384	WP_008361274.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2603935..2680259	76324
	-	flank	IS/Tn	-	-	2608293..2609648	1355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (98-214)

Antitoxin

• Antitoxin SocA-like, Panacea domain (113-225)

Sequences

Toxin

Protein

• Length: 236 a.a.
• Molecular weight: 28240.20 Da
• Isoelectric Point: 6.8881

>T144659 WP_157292747.1 NZ_CP046653:c2615049-2614342 [Bacillus sp. ms-22]
MINCTCELELFCESFFELINDINEAHKEISNEFKLEKKDELINLKRKQINHVTHVKEVNTFSVDNLFLQLISNYREKLIL
YAAYTDLGNKYRGLTLRSRTKETTSIINKLKYYLVGKKEEGKIPINKCLNDLFGFRIHPNNFDHNNECFQKTCQKLKQIY
NNRIDFMDSSKGEYKAFHIYLYGEEWSYFPWEIQIWCLTDLEKNNISHTQYKQEHRKWAEIYKNSTEIELEMEGV

Nucleotide

• Length: 708 bp

>T144659 NZ_CP062211:c2859223-2858972 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 250 a.a.
• Molecular weight: 28953.98 Da
• Isoelectric Point: 5.8008

>AT144659 WP_157292745.1 NZ_CP046653:c2614339-2613590 [Bacillus sp. ms-22]
MAFHFIAIVSDFSGGRRYGWHYASVQELDKEYIKLFMTRMKKECGHVQIGIHKLITDDTTWDSVVRMDSFFDDVIITNRI
DDFVEVVSNDQQLTAYDVAKFILAIYPVSHLKLQKLLYYIYSEFLIRTGSKLFKDPIVAFKYGPVVEDIFSRYRIHGSSQ
IDYKEDETFLLRTEEVAFTPSLIKIWTSEHGTAALDCIKDTLLKYGDLDAYTLVDKTHQSGGPWYRAYREGQNCAITDDL
IIQYHQYAT

Nucleotide

• Length: 750 bp

>AT144659 NZ_CP062211:c2858975-2858721 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA