Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 91276..91802 Replicon plasmid pEGKP3.3
Accession NZ_CP046647
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GPU01_RS27185 Protein ID WP_000323025.1
Coordinates 91515..91802 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GPU01_RS27180 Protein ID WP_000534858.1
Coordinates 91276..91515 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS27160 (GPU01_26765) 87604..88842 + 1239 WP_003030308.1 IS110 family transposase -
GPU01_RS27165 (GPU01_26770) 89318..89890 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
GPU01_RS27170 (GPU01_26775) 90090..91013 + 924 WP_020277922.1 cation diffusion facilitator family transporter -
GPU01_RS27175 91147..91251 - 105 Protein_113 hypothetical protein -
GPU01_RS27180 (GPU01_26785) 91276..91515 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GPU01_RS27185 (GPU01_26790) 91515..91802 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GPU01_RS27190 (GPU01_26795) 91869..92903 - 1035 Protein_116 IS481 family transposase -
GPU01_RS27195 92964..93056 + 93 Protein_117 Hok/Gef family protein -
GPU01_RS27200 93020..93121 + 102 Protein_118 DUF5431 family protein -
GPU01_RS27205 (GPU01_26805) 93286..93771 - 486 WP_020277937.1 hypothetical protein -
GPU01_RS27210 93961..94233 + 273 WP_023280873.1 hypothetical protein -
GPU01_RS27215 (GPU01_26810) 94230..94580 + 351 WP_020277938.1 hypothetical protein -
GPU01_RS27220 (GPU01_26815) 95097..95477 + 381 WP_015632488.1 hypothetical protein -
GPU01_RS27225 (GPU01_26820) 95543..95890 + 348 WP_020316654.1 hypothetical protein -
GPU01_RS27230 (GPU01_26825) 95978..96208 + 231 WP_020805755.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / qnrS1 / blaSHV-12 / aac(6')-Il / blaOXA-10 / qacE / sul1 - 1..154760 154760


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T144658 WP_000323025.1 NZ_CP046647:91515-91802 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T144658 NZ_CP062211:c2858975-2858721 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT144658 WP_000534858.1 NZ_CP046647:91276-91515 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT144658 NZ_CP062211:c2859223-2858972 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References