Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54634..55277 Replicon plasmid pEGKP3.3
Accession NZ_CP046647
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag GPU01_RS26960 Protein ID WP_032425563.1
Coordinates 54634..55050 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GPU01_RS26965 Protein ID WP_001261276.1
Coordinates 55047..55277 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS26945 (GPU01_26565) 49678..51786 - 2109 WP_265430391.1 peptidase domain-containing ABC transporter -
GPU01_RS26950 (GPU01_26570) 51776..53053 - 1278 WP_265430392.1 HlyD family secretion protein -
GPU01_RS26955 (GPU01_26575) 53715..54593 + 879 WP_009309916.1 restriction endonuclease -
GPU01_RS26960 (GPU01_26580) 54634..55050 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
GPU01_RS26965 (GPU01_26585) 55047..55277 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GPU01_RS26970 55330..55704 + 375 WP_171483593.1 hypothetical protein -
GPU01_RS26975 (GPU01_26590) 55865..56809 + 945 WP_011977810.1 hypothetical protein -
GPU01_RS26980 (GPU01_26595) 56846..57238 + 393 WP_011977811.1 hypothetical protein -
GPU01_RS26985 (GPU01_26600) 57296..57817 + 522 WP_013214008.1 hypothetical protein -
GPU01_RS26990 (GPU01_26605) 57863..58066 + 204 WP_011977813.1 hypothetical protein -
GPU01_RS26995 (GPU01_26610) 58096..59100 + 1005 WP_011977814.1 hypothetical protein -
GPU01_RS27000 (GPU01_26615) 59284..60063 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / qnrS1 / blaSHV-12 / aac(6')-Il / blaOXA-10 / qacE / sul1 - 1..154760 154760


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T144657 WP_032425563.1 NZ_CP046647:c55050-54634 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T144657 NZ_CP062211:2847079-2847453 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT144657 WP_001261276.1 NZ_CP046647:c55277-55047 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT144657 NZ_CP062211:2846622-2846990 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References