Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11120..11763 Replicon plasmid pEGKP3.1-2
Accession NZ_CP046646
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag GPU01_RS25795 Protein ID WP_016236302.1
Coordinates 11347..11763 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GPU01_RS25790 Protein ID WP_001261282.1
Coordinates 11120..11350 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS25765 (GPU01_25415) 6835..7092 - 258 WP_023292103.1 hypothetical protein -
GPU01_RS25770 7570..8298 + 729 Protein_6 CSS-motif domain-containing protein -
GPU01_RS25775 8314..9150 + 837 WP_032439674.1 EAL domain-containing protein -
GPU01_RS25780 (GPU01_25425) 9768..10199 - 432 WP_174805835.1 hypothetical protein -
GPU01_RS25785 10741..11163 - 423 WP_046624301.1 hypothetical protein -
GPU01_RS25790 (GPU01_25430) 11120..11350 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GPU01_RS25795 (GPU01_25435) 11347..11763 + 417 WP_016236302.1 type II toxin-antitoxin system VapC family toxin Toxin
GPU01_RS25800 (GPU01_25440) 11837..13399 + 1563 WP_004206609.1 AAA family ATPase -
GPU01_RS25805 (GPU01_25445) 13384..14406 + 1023 WP_032439672.1 helicase UvrD -
GPU01_RS25810 (GPU01_25450) 14951..15859 + 909 WP_117254255.1 HNH endonuclease signature motif containing protein -
GPU01_RS25815 (GPU01_25455) 16045..16395 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..162487 162487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15091.55 Da        Isoelectric Point: 7.1084

>T144654 WP_016236302.1 NZ_CP046646:11347-11763 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T144654 NZ_CP062211:c2006238-2006131 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT144654 WP_001261282.1 NZ_CP046646:11120-11350 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT144654 NZ_CP062211:2006286-2006353 [Escherichia coli]
GTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References