Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5266361..5266986 Replicon chromosome
Accession NZ_CP046645
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GPU01_RS25375 Protein ID WP_002882817.1
Coordinates 5266361..5266744 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GPU01_RS25380 Protein ID WP_004150355.1
Coordinates 5266744..5266986 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS25360 (GPU01_25010) 5263727..5264629 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GPU01_RS25365 (GPU01_25015) 5264626..5265261 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GPU01_RS25370 (GPU01_25020) 5265258..5266187 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GPU01_RS25375 (GPU01_25025) 5266361..5266744 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GPU01_RS25380 (GPU01_25030) 5266744..5266986 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GPU01_RS25385 (GPU01_25035) 5267191..5268108 + 918 WP_004895173.1 alpha/beta hydrolase -
GPU01_RS25390 (GPU01_25040) 5268122..5269063 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GPU01_RS25395 (GPU01_25045) 5269108..5269545 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GPU01_RS25400 (GPU01_25050) 5269542..5270402 - 861 WP_004146232.1 virulence factor BrkB family protein -
GPU01_RS25405 (GPU01_25055) 5270396..5270995 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T144653 WP_002882817.1 NZ_CP046645:c5266744-5266361 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T144653 NZ_CP062211:c2005702-2005595 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT144653 WP_004150355.1 NZ_CP046645:c5266986-5266744 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT144653 NZ_CP062211:2005755-2005816 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References